等离子体射流灭活液体中铜绿假单胞菌的研究

发布时间：2018-04-23 06:59

本文选题：等离子体射流 + 铜绿假单胞菌　；参考：《微生物学通报》2017年04期

【摘要】：【目的】测定等离子射流对铜绿假单胞菌(Pseudomonas aeruginosa)的灭活效果,探究低温等离子体射流的杀菌机理。【方法】采用平板计数法测定等离子体射流的杀菌效果,荧光显微镜、透射电镜观察等离子体作用后菌体结构的变化,蛋白浓度测定和SDS-PAGE电泳检测菌液上清液中可溶性蛋白的泄漏量。【结果】等离子体射流处理铜绿假单胞菌菌液5 min,杀灭率可达到99.9%以上。透射电镜观察可见细菌菌体结构发生改变,细胞壁、细胞膜损伤破裂,细胞内容物泄露。进一步对处理铜绿假单胞菌上清液中的蛋白质含量变化进行检测,结果显示随着处理时间的增加,上清液中蛋白质含量持续增加,在2 min时达到最大值。【结论】等离子体射流可以通过破坏细胞结构造成细胞质泄露,使其丧失正常的细胞功能,从而达到快速有效地杀灭铜绿假单胞菌的效果。
[Abstract]:[objective] to determine the inactivation effect of plasma jet on Pseudomonas aeruginosa and to explore the bactericidal mechanism of low temperature plasma jet. Transmission electron microscopy (TEM) was used to observe the changes of cell structure after plasma treatment. Protein concentration and SDS-PAGE electrophoresis were used to detect the leakage of soluble protein in the supernatant. [results] the killing rate of Pseudomonas aeruginosa solution treated with plasma jet for 5 min was over 99.9%. Transmission electron microscopy (TEM) showed that the bacterial cell structure, cell wall, cell membrane damage and rupture, leakage of cell contents. The protein content in the supernatant of Pseudomonas aeruginosa was detected. The results showed that the protein content in the supernatant increased with the increase of treatment time. The maximum value was reached at 2 min. [conclusion] Plasma jet can destroy the cell structure and cause the leakage of the cytoplasm, which can result in the loss of normal cell function, thus the rapid and effective killing effect of Pseudomonas aeruginosa can be achieved.
【作者单位】：塔里木大学生命科学学院新疆兵团塔里木盆地生物资源保护利用重点实验室;西安交通大学电力设备电气绝缘国家重点实验室等离子体生物医学研究中心;
【基金】：国家自然科学基金项目(No.51477136) 中央高校基本科研业务费项目~~
【分类号】：R378

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