免疫组化法观察日本血吸虫感染破坏小鼠脾脏淋巴滤泡结构

发布时间：2018-04-25 23:14

本文选题：日本血吸虫 + 免疫组化　；参考：《中国血吸虫病防治杂志》2017年04期

【摘要】：目的采用免疫组化(IHC)方法观察日本血吸虫感染破坏小鼠脾脏淋巴滤泡结构的现象。方法日本血吸虫感染小鼠(20条尾蚴/鼠)8周后剖杀,取脾脏样本石蜡包埋、切片,先以苏木素-伊红(HE)染色观察小鼠脾脏淋巴滤泡整体结构,再以IHC法用抗B220、CD21及Ki67抗体标记淋巴滤泡内B细胞、滤泡树突状细胞(FDC)及生发中心细胞,观察滤泡内具体细胞分布。结果 HE染色显示,感染日本血吸虫小鼠脾脏淋巴滤泡结构模糊难辨,相较于正常小鼠滤泡数目及面积均显著减少;IHC染色发现,日本血吸虫感染小鼠脾脏T、B淋巴细胞分区及FDC网络结构和生发中心结构均消失。结论日本血吸虫感染可显著破坏小鼠脾脏淋巴滤泡结构。
[Abstract]:Objective to observe the effect of Schistosoma japonicum infection on the structure of splenic lymphatic follicles in mice by immunohistochemical staining (IHC). Methods Twenty cercariae / mice infected by Schistosoma japonicum were dissected after 8 weeks. The spleen samples were embedded in paraffin and sectioned. The whole structure of lymphoid follicles was observed by hematoxylin and eosin (HEH) staining. B cells in lymphoid follicles, follicular dendritic cells (DC) and germinal center cells were labeled with anti-B220 CD21 and Ki67 antibodies by IHC method. The distribution of specific cells in follicles was observed. Results HE staining showed that the structure of splenic lymphatic follicles in Schistosoma japonicum infected mice was fuzzy and difficult to distinguish. Compared with normal mice, the number and area of follicles were significantly decreased by IHC staining. In mice infected with Schistosoma japonicum, the distribution of T _ (B) lymphocytes, the network structure of FDC and the structure of germinal center disappeared. Conclusion Schistosoma japonicum infection can significantly destroy the lymphatic follicular structure in mice.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所卫生部寄生虫病原与媒介生物学重点实验室世界卫生组织热带病合作中心科技部国家级热带病国际联合研究中心;
【基金】：国家自然科学基金(81371841) 上海市三年行动计划第四轮重点学科(15GWZK0101)
【分类号】：R383.24

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