干细胞移植对大脑中动脉闭塞模型鼠脑组织miR-34a及survivin表达的影响

发布时间：2018-04-26 00:38

本文选题：脑缺血-再灌注 + 骨髓间充质干细胞　；参考：《国际神经病学神经外科学杂志》2016年02期

【摘要】：目的观察骨髓间充质干细胞(BMSCs)移植对缺血再灌注损伤后大鼠脑组织miR-34a和survivin表达的影响,探讨BMSCs移植的抗凋亡和神经保护作用机制。方法将192只大鼠随机分为空白组、模型组、PBS液移植组和干细胞移植组;采用改良Longa线栓法制作大鼠大脑中动脉闭塞再灌注(MCAO)模型;通过尾静脉注射法行干细胞移植;改良大鼠神经功能缺损评分(m NSS)评估神经功能缺损;免疫组化检测survivin的表达;实时荧光定量PCR技术检测miR-34a的表达。结果干细胞移植组的神经功能缺损评分在12 h、1 d时与模型组比较无明显差异(P0.05);3 d、7 d时明显低于模型组(P0.05)。干细胞移植组的survivin阳性细胞率在各时间点均显著高于模型组(P0.05)。干细胞移植组的miR-34 a表达量在各时间点均显著低于模型组(P0.01)。结论大鼠脑缺血-再灌注损伤可致病灶区miR-34 a的表达上调;干细胞移植可明显改善脑缺血-再灌注大鼠的神经功能;移植干细胞可能通过下调病灶区miR-34a和上调survivin的表达发挥抗凋亡及神经保护作用。
[Abstract]:Objective to observe the effect of bone marrow mesenchymal stem cell transplantation (BMSCs) on the expression of miR-34a and survivin in rat brain after ischemia-reperfusion injury and to explore the mechanism of anti-apoptosis and neuroprotective effect of BMSCs transplantation. Methods 192 rats were randomly divided into blank group (model group) and stem cell transplantation group (MCAO) model of middle cerebral artery occlusion (MCAO) was established by modified Longa thread occlusion (MCAO) method, and stem cell transplantation was performed by caudal vein injection. The neural function defect was evaluated by modified neurologic impairment score (survivin), the expression of survivin was detected by immunohistochemistry, and the expression of miR-34a was detected by real-time fluorescence quantitative PCR. Results there was no significant difference in neurological deficit score between the stem cell transplantation group and the model group at 12 h and 1 d after transplantation compared with the model group. The score of neural function defect in the stem cell transplantation group was significantly lower than that in the model group at 3 days and 7 days. The percentage of survivin positive cells in stem cell transplantation group was significantly higher than that in model group at each time point. The expression of miR-34 a in stem cell transplantation group was significantly lower than that in model group at each time point. Conclusion the expression of miR-34 a in focal area can be up-regulated by cerebral ischemia-reperfusion injury in rats, and the neural function of rats with cerebral ischemia-reperfusion can be improved by stem cell transplantation. Transplantation of stem cells may play an antiapoptotic and neuroprotective role by down-regulating miR-34a and up-regulating the expression of survivin.
【作者单位】：南华大学附属第二医院神经内科;
【基金】：湖南省科技厅计划项目(2012FJ3104)
【分类号】：R743;R-332

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