补肾填精方对去卵巢骨质疏松模型大鼠胶原代谢的影响

发布时间：2018-04-26 02:16

  本文选题：骨质疏松 + 补肾填精　； 参考：《时珍国医国药》2017年04期

【摘要】：目的通过对比观察补肾填精方左归丸和左归丸去补阳药对去卵巢骨质疏松模型大鼠胶原代谢的影响,探讨左归丸阳中求阴法防治骨质疏松症的作用机制。方法取雌性SD大鼠32只,采用双侧卵巢摘除术建立骨质疏松模型,随机分为假手术组(Sham)、模型对照组(OVX)、左归丸组(ZGP)、左归丸去补阳药组(ZGPQ),每组8只。灌胃给药12周后收集各组大鼠血清和股骨组织,采用ELISA法检测血清中PICP和ICTP水平,采用Masson染色法检测股骨组织胶原纤维形态,采用免疫组织化学染色法检测股骨组织ColⅠ蛋白表达水平。结果与Sham比较,OVX血清PICP和ICTP水平显著升高(P0.01)、股骨组织ColⅠ蛋白表达显著降低;与OVX比较,补肾填精方下调血清PICP和ICTP水平(P0.05,P0.01)、上调股骨组织ColⅠ蛋白表达。结论补肾填精方左归丸对去卵巢引起的骨质疏松症具有一定的防治作用,其作用机制可能与其干预胶原代谢有关,全方效果较为明显。
[Abstract]:Objective to observe the effects of Zuo Gui Pill and Zuo Gui Pill on Collagen Metabolism in Ovariectomized Ovariectomized Rats, and to explore the mechanism of preventing and treating osteoporosis by Zuo Gui Pill. Methods Osteoporosis model was established in 32 female SD rats by bilateral ovariectomy. The model rats were randomly divided into sham-operated group (OVX), model control group (OVX), Zogui pill group (ZGPGX), Zogui pill group (ZGPQN), each group (8 rats). The serum and femur tissues were collected 12 weeks after intragastric administration, the levels of PICP and ICTP in serum were detected by ELISA method, and the morphology of collagen fibers in femur tissue were detected by Masson staining. Immunohistochemical staining was used to detect the expression of Col 鈪，

本文编号：1804032