骨髓间充质干细胞联合外源性Shh蛋白对骨髓造血干细胞增殖的影响

发布时间：2018-04-26 02:36

本文选题：骨髓造血干细胞 + 骨髓间充质干细胞　；参考：《山东医药》2017年37期

【摘要】：目的探讨骨髓间充质干细胞(BMMSC)联合外源性Shh蛋白干预对骨髓造血干细胞(HSC)增殖的影响。方法分别采用全骨髓培养法、磁珠分选法(MASC)从正常志愿者骨髓中分离BMMSC、HSC,并用流式细胞术进行鉴定。将分选好的HSC随机分为三组,MSC+HSC+Shh组Transwell培养板上室接种用IMDM培养基+500ng/m L的Shh蛋白重悬的HSC,下室接种BMMSC;BMMSC+HSC组上室接种用IMDM培养基重悬后的HSC,下室接种BMMSC;HSC+Shh组上室接种用IMDM培养基+500 ng/mL的Shh蛋白重悬的HSC,下室加入培养基;对照组上室接种用IMDM培养基重悬的HSC,下室加入培养基。各组均培养72 h,用计数板计数HSC活细胞数。结果培养72 h,HSC+BMMSC+Shh组HSC细胞计数较其他各组高(P均0.05),HSC+MSC组、HSC+Shh组较HSC组高(P均0.05),HSC+BMMSC组、HSC+Shh组比较差异无统计学意义(P0.05)。结论 BMMSC与外源性Shh蛋白可协同作用共同促进HSC增殖。
[Abstract]:Objective to investigate the effects of bone marrow mesenchymal stem cells (BMMSC) combined with exogenous Shh protein on the proliferation of bone marrow hematopoietic stem cells (HSCs). Methods BMMSC-HSCs were isolated from the bone marrow of normal volunteers by whole bone marrow culture method and magnetic bead sorting method respectively, and were identified by flow cytometry. The selected HSC were randomly divided into three groups: the Transwell HSC Shh group was inoculated with IMDM medium 500ng/m L Shh protein, the lower chamber group was inoculated with the IMDM medium, and the lower chamber group was inoculated with the IMDM medium 500ng/m L, and the lower chamber was inoculated with the upper chamber of the IMDM medium 500ng/m L, the lower chamber inoculated with the IMDM medium, and the lower chamber inoculated with the upper chamber of the BMMSC-HSC Shh group. The IMDM medium with 500 ng/mL Shh protein was inoculated and the lower chamber was added to the medium. In the control group, the upper chamber was inoculated with IMDM medium and the lower chamber was added to the medium. All groups were cultured for 72 hours and counted the number of HSC living cells with counter. Results the number of HSC cells in HSC BMMSC Shh group was higher than that in other groups (P < 0.05). There was no significant difference between HSC Shh group and HSC group (P 0.05). Conclusion BMMSC and exogenous Shh protein can promote the proliferation of HSC.
【作者单位】：西南医科大学附属医院;
【分类号】：R329.2

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