qRT-PCR检测结核分枝杆菌毒素-抗毒素系统mazE F的表达

发布时间：2018-04-26 03:27

本文选题：结核分枝杆菌 + 毒素-抗毒素系统　；参考：《中国人兽共患病学报》2017年02期

【摘要】：目的探讨结核分枝杆菌毒素基因mazF3,6,9及抗毒素基因mazE3,6,9的表达差异。方法运用实时定量PCR检测结核分枝杆菌单耐药株20株,耐多药株20株和标准株H37Rv毒素基因mazF3,6,9及抗毒素基因mazE3,6,9的表达水平;组间基因表达水平的差异用one-way ANOVA进行统计分析。结果与对照株相比较,毒素基因mazF6,9在单耐药组(11.151 9±22.317 21;8.430 6±17.978 97)及耐多药组(4.601 6±1.290 18;6.962 7±6.929 48)表达均上调且差异有统计学意义(P0.01),mazF3基因在单耐药组及耐多药组差异无统计学意义(P0.05),抗毒素基因mazE3在单耐药(0.360 6±0.125 27)及耐多药组(0.201 6±0.165 42)中表达均下调,差异有统计学意义(P0.01),mazE6均无统计学意义,mazE9只有在耐多药组(0.398 9±0.376 79)中表达下调,差异有统计学意义,(P0.01)。结论毒素基因mazF6,9抗毒素基因mazE3,9可能参与了结核分枝杆菌的耐药形成,具体机制尚待进一步的研究。
[Abstract]:Objective to investigate the difference between the expression of Mycobacterium tuberculosis toxin gene mazF3O6O9 and the anti-toxin gene mazE3O6O9. Methods Real-time quantitative PCR was used to detect the expression levels of the H37Rv toxin gene mazF3O6N9 and the anti-toxin gene mazE3O6N9 in 20 strains of mycobacterium tuberculosis, 20 strains of multidrug resistance and 20 strains of standard strains, and the difference of gene expression between groups was analyzed by one-way ANOVA. Results compared with the control plant, The expression of the toxin gene mazF6n9 was up-regulated in the single drug resistance group (11.1559 卤22.317 21) and the multidrug resistance group (4.6016 卤1.290 186.9627 卤6.929 48), and there was no significant difference in the expression of the toxin gene maz F3 gene in the single drug resistance group and the multidrug resistance group (P 0.05), while the mazE3 of the antitoxin gene was not significantly different in the single drug resistance group and the multidrug resistance group. The expression of YD in 0.360 6 卤0.125 27) and multidrug resistant group (0.201 6 卤0.165 42) was down-regulated. There was no significant difference in the expression of maz E9 in multidrug resistant group (P 0.01), and the difference was statistically significant (P 0.01) only in the multidrug resistant group (0.398 9 卤0.376 79). Conclusion the anti-toxin gene mazE3O9 may be involved in the formation of drug resistance of Mycobacterium tuberculosis, and the specific mechanism remains to be further studied.
【作者单位】：石河子大学医学院免疫学教研室;
【基金】：国家自然科学基金(No.81341079,81560264)资助~~
【分类号】：R378.911

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