调节性T细胞和Th17细胞在新型小鼠重型再生障碍性贫血模型中的变化

发布时间：2018-04-26 04:37

本文选题：再生障碍性 + 动物模型　；参考：《南通大学》2012年硕士论文

【摘要】：目的探讨调节性T细胞和Th17细胞在干扰素-γ(interferon-γ, IFN-γ)联合白消安诱导的新型小鼠重型再生障碍性贫血(再障)模型中的变化,从免疫学水平上论证该模型合理性。方法应用IFN-γ腹腔注射联合白消安灌胃诱导建立BALB/c小鼠重型再障模型(联合组,n=15),以白消安组(n=15)、IFN-γ组(n=15)和正常组(n=15)为对照,分别收集各组小鼠的外周血和脾脏中的单个核细胞,采用流式细胞术检测各组小鼠外周血和脾脏中CD4+CD25+Foxp3+调节性T细胞(Treg)和Th17细胞的比例变化,并逐层分析比较。结果联合组小鼠外周血和脾脏的调节性T细胞的百分比分别为(13.19±0.76)%和(4.77±1.05)%,较正常组、白消安组、IFN-γ组显著降低(P值均0.()1)：而Th17细胞百分比分别为(2.07±0.12)%和(3.18±0.46)%,较其他三组均显著升高(P值分别0.05和0.01)。结论IFN-γ联合白消安诱导建立的重型再障小鼠调节性T细胞数量降低而Th17细胞升高,提示该小鼠重型再障模型可能更接近并模拟了人类免疫介导的骨髓造血功能损伤,可以为今后的实验提供可能的理论依据。
[Abstract]:Objective to investigate the changes of regulatory T cells and Th17 cells in a new mouse model of severe aplastic anemia (aplastic anemia) induced by interferon- 纬 (IFN- 纬) and oxacol, and to demonstrate the rationality of the model from the immunological level. Methods the model of severe aplastic anemia in BALB/c mice was established by intraperitoneal injection of IFN- 纬 and oral administration of Paixiaan. Mononuclear cells were collected from peripheral blood and spleen of BALB/c mice. The percentage of CD4 CD25 Foxp3 regulatory T cells and Th17 cells in peripheral blood and spleen of each group were detected by flow cytometry. Results the percentages of regulatory T cells in peripheral blood and spleen of the combined group were 13.19 卤0.76% and 4.77 卤1.05%, respectively, which were higher than those in the normal group. The percentage of Th17 cells were 2.07 卤0.12% and 3.18 卤0.46%, respectively, which were significantly higher than those of the other three groups. Conclusion the number of regulatory T cells decreased and Th17 cells increased in mice with severe aplastic anemia induced by IFN- 纬 and oxacol, suggesting that the model of severe aplastic anemia in mice may be closer to and mimic the human immune-mediated hematopoietic impairment of bone marrow. It can provide possible theoretical basis for future experiments.
【学位授予单位】：南通大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R556.5;R-332

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