血小板低下对树突状细胞在体迁移与归巢模式的影响研究

发布时间：2018-04-26 05:19

  本文选题：血小板低下 + 树突状细胞　； 参考：《中国实验血液学杂志》2017年01期

【摘要】：目的:探讨血小板低下对过继性树突状细胞(DC)在体内迁移模式的影响。方法:腹腔注射抗CD41单克隆抗体以建立血小板低下小鼠模型。将带报告基因的骨髓来源DC经脚垫和静脉两种途径输注至血小板低下和正常小鼠体内,用活体成像技术监测DC在小鼠体内的迁移和组织内分布情况。结果:经抗GPⅡb抗体处理组小鼠体内80%以上血小板被清除。DC经脚垫回输72 h后,在血小板低下组迁移到乆窝淋巴结(PLN)和腹股沟淋巴结(ILN)的比例分别为(0.32±0.02)%和(0.02±0.01)%;对照组上述两部位内为(0.27±0.15)%和(0.02±0.02)%。两组之间无统计学差异(P0.05)。经静脉输注DC 72 h后,血小板低下组和对照组小鼠组织内的分布均主要集中在脾脏、肝脏引流淋巴结、肺脏和肝脏等,且分布比例无统计学差异。结论:小鼠体内血小板低下至不足20%时,对经皮下和静脉两种途径输注的DC在体内迁移和组织分布无明显影响。
[Abstract]:Aim: to investigate the effect of thrombocytopenia on the migration pattern of adoptive dendritic cells (DC) in vivo. Methods: mouse model of thrombocytopenia was established by intraperitoneal injection of anti-CD41 monoclonal antibody. DC derived from bone marrow with reporter gene was infused into mice with thrombocytopenia and normal mice via foot pad and vein. The migration and distribution of DC in vivo were monitored by in vivo imaging. Results: more than 80% of platelets were cleared in mice treated with anti-GP 鈪，

本文编号：1804657