尿酸盐致人血管内皮细胞炎性损伤机制的研究

发布时间：2018-04-26 07:45

本文选题：内皮细胞 + 高尿酸血症　；参考：《青岛大学》2012年硕士论文

【摘要】：目的：通过观察人血管内皮细胞在不同浓度尿酸盐刺激后,相关炎症因子表达的变化,探讨尿酸盐对人血管内皮细胞炎性损伤的机制。方法：体外培养人血管内皮细胞,分别用含尿酸盐浓度为720μ mol/L(高2)、540u mo1/L(高)、420μmol/L(中)、300μ mol/L(低1)、180μ mol/L(低2)的培养液刺激24h,对照组不加尿酸她刺激,用酶联免疫吸附双抗体夹心法(ELISA)测定上清液单核细胞趋化蛋白-1(MCP-1)、细胞间粘附分子-1(ICAM-1)、血管细胞粘附分子-1(VCAM-1)表达水平,用逆转录-聚合酶链式反应(RT-PCR)技术测定上述3种因子mRNA的表达水平。结果：①MCP-1：上清液中对照组与高2、高1浓度组比较,结果有显著差异(P＜0.05),中浓度组与高2浓度组比较,结果有显著差异(P＜0.05),高2与高1浓度组比较,结果有显著差异(P＜0.05),mRNA水平上对照组与高2、高1、中浓度组比较结果有显著差异(P＜0.05)；②ICAM-1:上清液中对照组与高2、高1浓度组比较,结果有显著差异(P＜0.05),中浓度组与高2、高1浓度组比较,结果有显著差异(P＜0.05),mRNA水平上对照组与高2、高1、中浓度组比较结果有显著差异(P＜0.05)；③VCAM-1:土青液和mRNA水平对照组与其他各组比较均无显著差异(P＞0.05)。结论：尿酸盐可以直接激活内皮细胞,引起相关炎症因子mRNA及蛋白水平的升高,诱发炎症反应。
[Abstract]:Aim: to investigate the mechanism of inflammatory injury induced by uric acid in human vascular endothelial cells (HUVEC) by observing the expression of related inflammatory factors after stimulation with different concentrations of uric acid. Methods: human vascular endothelial cells were cultured in vitro. The cultured human vascular endothelial cells were stimulated with 720 渭 mol / L uric acid at a concentration of 720 渭 mol / L (540u / L) (420 渭 mol / L) (300 渭 mol / L) (180 渭 mol / L (low 2) for 24 hours, while those in the control group were not stimulated by uric acid. Enzyme linked immunosorbent assay (Elisa) was used to detect the expression of monocyte chemoattractant protein (MCP-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule (VCAM-1) in supernatant. Reverse transcription-polymerase chain reaction (RT PCR) was used to detect the expression of mRNA. Results in the supernatant, there were significant differences between the control group and the high concentration group (P < 0.05), the middle concentration group compared with the high 2 concentration group (P < 0.05), the high 2 concentration group compared with the high concentration group (P < 0.05), and the high 2 group compared with the high concentration group (P < 0.05). Results there was significant difference in mRNA level between the control group and the control group (P < 0.05). The results of the middle concentration group were significantly higher than those of the control group (P < 0.05) and the high concentration group (P < 0.05). The results of the supernatant medium group were significantly different from those of the control group (P < 0.05) and the high concentration group (P < 0.05), and the comparison of the middle concentration group with the high concentration group (2%) and the high concentration group (1) showed significant difference (P < 0.05). Results there was significant difference in mRNA level between the control group and the control group (P < 0.05). There was significant difference between the control group and the control group (P < 0.05). There was no significant difference between the control group and other groups in the level of Tu Qing liquid and mRNA (P > 0.05). Conclusion: uric acid can directly activate endothelial cells, increase the level of mRNA and protein, and induce inflammatory reaction.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

【共引文献】