昆明种乳鼠肝细胞原代培养方法的比较研究

发布时间：2018-04-29 23:30

本文选题：肝细胞 + 乳鼠　；参考：《安徽医学》2016年01期

【摘要】：目的通过比较3种昆明鼠乳鼠肝细胞原代培养的方法,探索一种更有效、快捷的原代培养方法。方法取30只1~3日龄昆明种乳鼠,随机分为3组,分别采用组织块培养法、胰酶消化法、组织块联合胰酶消化法进行肝细胞原代培养,用倒置显微镜观察实验过程中肝细胞的形态及生长情况。结果 3种方法均能进行乳鼠肝细胞的原代培养,其中组织块联合胰酶消化法培养的肝细胞形态学和生物学特性优于其他两种方法培养的细胞。结论组织块联合胰酶消化法是3种方法中最快速和高效的一种方法,获得的乳鼠肝细胞形态及生物学特性好,为体外建立乳鼠肝细胞实验模型奠定了实验基础。
[Abstract]:Objective to explore a more effective and rapid primary culture method by comparing three methods of primary culture of mouse hepatocytes. Methods Thirty Kunming mice of 1 and 3 days old were randomly divided into 3 groups. The primary culture of hepatocytes was carried out by tissue mass culture, trypsin digestion and tissue mass combined with trypsin digestion. The morphology and growth of hepatocytes were observed by inverted microscope. Results all the three methods could be used for primary culture of rat hepatocytes. The morphological and biological characteristics of hepatocytes cultured by tissue mass combined with trypsin digestion were superior to those of the other two methods. Conclusion the method of tissue mass combined with trypsin digestion is the fastest and most efficient method among the three methods. The obtained hepatocytes have good morphology and biological characteristics, which lays an experimental foundation for the establishment of experimental model of neonatal rat hepatocytes in vitro.
【作者单位】：川北医学院临床医学系;川北医学院组织工程与干细胞研究所、南充市中心医院生物治疗中心;川北医学院基础医学院生物学教研室;
【基金】：四川省教育厅科研项目(项目编号:12ZB046) 四川省卫生厅科研项目(项目编号:120457) 南充市科技支撑计划项目(项目编号:2010SF07) 川北医学院科研发展计划项目(项目编号:CBY11-A-2P14)
【分类号】：R-33

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