猪带绦虫重组BCG-TSOL18疫苗诱导仔猪的保护性免疫应答

发布时间：2018-05-01 14:13

本文选题：猪带绦虫 + 重组BCG-TSOL疫苗　；参考：《现代免疫学》2017年05期

【摘要】：研究猪带绦虫重组BCG-TSOL18疫苗免疫仔猪,猪带绦虫卵攻击后诱导其保护性免疫应答的情况。将12头40d龄健康仔猪均分为3组:分别为1011 CFU重组BCG-TSOL18疫苗灌胃组、BCG对照组和PBS对照组。每2周免疫1次,共免疫2次。于末次免疫4周后用猪带绦虫卵进行攻击感染,3月后剖杀仔猪,分离囊尾蚴,计算减蚴率;取前腔静脉血,分离血清和制备外周血单个核细胞(peripheral blood monouclear cell,PBMC)。采用ELISA法检测血清IgG、IgG1及IgG2a水平以及PBMC培养上清液中IL-2、IFN-γ、IL-4及IL-10的水平;MTT比色法检测PBMC增殖水平。重组BCG-TSOL18疫苗免疫组猪带绦虫卵攻击后诱导仔猪获得79.44%的保护力。与BCG和PBS对照组比较,重组BCG-TSOL18疫苗组血清IgG、IgG2a水平显著升高(t=13.026,P=0.000;t=13.253,P=0.000;t=7.284,P=0.000;t=8.453,P=0.000),IgG1水平显著降低(t=-9.118,P=0.000;t=-10.372,P=0.000);抗原刺激下PBMC显著增殖(t=14.929,P=0.000;t=14.665,P=0.000);PBMC培养上清液IL-2、IFN-γ水平显著升高(t=9.768,P=0.000;t=10.496,P=0.000;t=22.504,P=0.000;t=23.204,P=0.000),IL-4和IL-10水平显著降低(t=-18.659,P=0.000;t=-19.495,P=0.000;t=-26.258,P=0.000;t=-27.402,P=0.000)。猪带绦虫重组BCG-TSOL18疫苗可诱导仔猪产生Th1型的保护性免疫应答,从而抵抗猪带绦虫卵的感染攻击。
[Abstract]:To study the protective immune response induced by the recombinant BCG-TSOL18 vaccine of Taenia solium and the egg attack of Taenia solium, 12 40d age healthy piglets were divided into 3 groups: 1011 CFU recombinant BCG-TSOL18 vaccine group, BCG control group and PBS control group, 1 times every 2 weeks and 2 times in CO immunization. The pigs were used for 4 weeks after the last immunization. The eggs of the tapeworm were infected. After March, the piglets were killed, the cysticercus was separated and the rate of Echinococcus was calculated; the blood of the anterior vena cava, the serum and the peripheral blood mononuclear cells (peripheral blood monouclear cell, PBMC) were obtained. The level of IgG, IgG1 and IgG2a in the serum and IL-2, IFN- gamma, and the levels of PBMC were detected by ELISA. The proliferation level of PBMC was detected by colorimetric method. The recombinant BCG-TSOL18 Vaccine Immunized with Taenia solium eggs to induce 79.44% protection. Compared with the control group of BCG and PBS, the serum IgG and IgG2a level of the recombinant BCG-TSOL18 vaccine group increased significantly (t=13.026, P=0.000; t=13.253, P= 0; Low (t=-9.118, P=0.000; t=-10.372, P=0.000); PBMC proliferation under the stimulation of antigen (t=14.929, P=0.000; t=14.665, P=0.000); PBMC culture supernatant IL-2, IFN- gamma level significantly increased 8, P=0.000; t=-27.402, P=0.000). Recombinant BCG-TSOL18 vaccine of Taenia solium can induce Th1 type protective immune response in piglets, thus resisting the infection attack of Taenia solium eggs.

【作者单位】：遵义医学院寄生虫学教研室;遵义医学院附属医院中医肛肠科;
【基金】：贵州省科技厅社会发展攻关项目(黔科合SY字[2011]3038号)
【分类号】：R392

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