山茱萸多糖对阿尔茨海默病模型大鼠海马区糖原合酶激酶-3β和磷酸化-tau蛋白的影响

发布时间：2018-05-01 16:26

本文选题：山茱萸多糖 + 阿尔茨海默病　；参考：《中国老年学杂志》2017年12期

【摘要】：目的观察山茱萸多糖(CFPs)对阿尔茨海默病(AD)大鼠海马区糖原合酶激酶(GSK)-3β和磷酸化tau(p-tau)蛋白的影响,探讨CFPs对AD大鼠神经元的保护机制。方法将Wistar大鼠100只随机分为青年组、假手术组、模型组、多奈哌齐组、CFPs组。采用D-半乳糖联合Aβ1~40制备AD模型,采用免疫组化(SABC)法检测海马CA1区GSK-3β和p-tau蛋白表达量,Western印迹法检测海马GSK-3β和p-tau蛋白表达量。结果与模型组比较,CFPs和多奈哌齐可显著降低AD大鼠海马区GSK-3β和p-tau蛋白表达量(P0.05),CFPs组和多奈哌齐组GSK-3β和ptau蛋白表达量无显著差异(P0.05)。结论 CFPs通过抑制大鼠海马区GSK-3β蛋白表达从而降低tau蛋白过度磷酸化,实现其对AD大鼠神经元的保护作用。
[Abstract]:Objective To observe the effect of Fructus Corni polysaccharide (CFPs) on the glycogen synthase kinase (GSK) -3 beta (GSK) -3 beta and phosphorylated tau (p-tau) protein in the hippocampus of Alzheimer's disease (AD) rats and to explore the protective mechanism of CFPs on AD rats. Methods 100 Wistar rats were randomly divided into young group, sham operation group, model group, donepezil group, CFPs group. The AD model was prepared with A beta 1~40. The expression of GSK-3 beta and p-tau protein in hippocampus CA1 region was detected by immunohistochemistry (SABC). The expression of GSK-3 beta and p-tau protein in hippocampus was detected by Western blotting. There was no significant difference in the expression of GSK-3 beta and PtAu protein in the group (P0.05). Conclusion CFPs can reduce the excessive phosphorylation of tau protein by inhibiting the expression of GSK-3 beta protein in the hippocampus of rats, so as to realize its protective effect on the neuron of AD rat.

【作者单位】：佳木斯大学附属第一医院;佳木斯大学基础医学院;
【基金】：黑龙江省卫生厅项目(2013-233) 佳木斯大学面上项目(L2012-052) 佳木斯大学附属第一医院培育课题(jy2014-005)
【分类号】：R285.5;R-332

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