红内期恶性疟原虫连续体外培养中污染细菌鉴定与实验室空气质量控制措施

发布时间：2018-05-03 00:13

本文选题：恶性疟原虫 + 体外培养　；参考：《大理学院》2011年硕士论文

【摘要】：目的1.观察鉴定体外培养疟原虫过程中容易污染的细菌种类、来源及药物敏感性,初步探寻控制污染的方法。2.探寻紫外线照射和甲醛熏蒸实验室内空气消毒效果。方法在采用CO2混合气体法人工培养恶性疟原虫过程中,对被污染的培养物进行细菌鉴定；对操作者手指部进行表面采样法培养鉴定。采用每天84消毒液拖地、新洁尔灭擦拭门和操作台,延长实验室紫外线辐射和操作前新洁尔灭洗手、操作中物体表面喷洒酒精等措施进行消毒控制。对实验室内空气采用自然沉降法采样培养鉴定；实验室内甲醛熏蒸前和之后每隔24h进行空气采样1次,连续进行1周,间隔一定时期后重复上述采样过程。结果培养过程中培养物污染的细菌为嗜麦芽寡养单胞菌Sernotrphomonas maltophilia、鲍曼氏/醋酸钙不动杆菌复合体A. baumannii / calcoaceticus complex和鲁氏不动杆菌Acinetobacter lwoffii (acilwo),均为革兰阴性杆菌(G-b),且属于多重耐药菌株；实验室不同时期空气中细菌构成不同,初期检测发现以杆菌为主,如假单胞菌Pseudomonas、鲍曼氏不动杆菌A. baumannii、阴沟肠杆菌E. cloacae等,后期检测发现主要为革兰阳性球菌(G+c),特别是藤黄微球菌M. luteus和解糖葡萄球菌S. saccharolyticus,均对庆大霉素敏感；手部携带菌株为常见的表皮葡萄球菌S. epidermidi和藤黄微球菌M. luteus。甲醛熏蒸和增加紫外线照射时间均能够减少空气中细菌数量。结论通过加强消毒措施同时应用庆大霉素可以很好地控制体外培养疟原虫时出现的细菌污染。影响紫外线照射效果因素极多,并且影响自然沉降法空气采样结果之因素亦较多,紫外线照射时间与空气中细菌数量之间的曲线不易准确绘出。甲醛熏蒸能够很好减少空气中细菌数量,但随着时间延长效果逐步减弱,且熏蒸后24～48h内气味较重,影响工作人员身体。
[Abstract]:Objective 1. To observe and identify the species, sources and drug sensitivity of bacteria liable to contamination during the culture of Plasmodium in vitro, and to explore the method of pollution control. 2. To explore the effect of ultraviolet irradiation and formaldehyde fumigation in laboratory air disinfection. Methods in the process of artificial culture of Plasmodium falciparum by CO2 mixed gas method, the contaminated cultures were identified, and the surface samples of the operator's fingers were cultured and identified. Every day 84 disinfectant was used to mop the ground, Xinjier wiped the door and the operating table, prolonged the ultraviolet radiation in the laboratory, washed hands before the operation, and sprayed alcohol on the surface of the object in operation to carry on the disinfection control. The air in the laboratory was sampled and identified by natural sedimentation method, and the air was sampled once every 24 hours before and after formaldehyde fumigation in the laboratory, and the sampling process was repeated after a certain interval for one week. Results the bacteria contaminated by culture were Sernotrphomonas maltophilia, Acinetobacter baumannii / calcium Acinetobacter complex A. baumannii / calcoaceticus complex and Acinetobacter rubrum Acinetobacter lwoffii. The composition of bacteria in the air was different in different periods of the laboratory. The main bacteria were Pseudomonas pseudomonas, Acinetobacter baumannii, Enterobacter cloacae E. cloacae, and so on. It was found that Gram-positive cocci, especially Micrococcus luteus and S.saccharolyticus, were sensitive to gentamicin, and the common strains were S. epidermidis epidermidi and M. luteus. Formaldehyde fumigation and increasing UV exposure time can reduce the number of bacteria in the air. Conclusion Gentamicin can effectively control the bacterial contamination of Plasmodium falciparum cultured in vitro by strengthening disinfection measures at the same time. There are many factors that influence the effect of ultraviolet radiation, and there are also many factors that affect the air sampling results by natural sedimentation method. The curve between ultraviolet irradiation time and bacteria quantity in air is not easy to draw accurately. Formaldehyde fumigation could reduce the number of bacteria in the air, but the effect of formaldehyde fumigation decreased gradually with the prolongation of time, and the smell of formaldehyde fumigation was more serious within 24 ~ 48 hours after fumigation, which affected the health of the workers.
【学位授予单位】：大理学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R378

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