淫羊藿苷元微乳制剂对小鼠免疫性血小板减少性紫癜模型的治疗作用

发布时间：2018-05-03 00:11

本文选题：免疫性血小板减少性紫癜 + 淫羊藿苷元微乳制剂　；参考：《中国新药杂志》2017年05期

【摘要】：目的:研究淫羊藿苷元对小鼠免疫性血小板减少性紫癜(immunologic thrombocytopenic purpura,ITP)模型的治疗作用。方法:雄性BALB/c小鼠随机分为5组,除正常对照组外,从给药d 1开始至d 9,小鼠每隔1 d腹腔注射豚鼠抗小鼠血小板血清(1∶4,100μL)诱导小鼠长期ITP。分离BALB/c小鼠血小板皮下注射至豚鼠体内,连续5周,制备豚鼠抗小鼠血小板血清。4组ITP模型小鼠分别灌胃(ig)给药10 d:微乳基质、重组人血小板生成素(recombinant human thrombopoietin,rh TPO)3 000 U·kg-1、淫羊藿苷元微乳制剂(Y003:1.25和5.0 mg·kg-1),空白对照组给予微乳基质。d 11处死动物,取血、股骨、脾脏,并测定以下指标:血常规、脏体比、流式网织血小板计数、ELISA测定血清中血小板生成素(thrombopoietin,TPO)含量、脾脏病理学检测。结果:1.25 mg·kg-1剂量给药组与模型组比较,外周血小板数、网织血小板比例、脏体比、血清TPO含量等指标均有显著差异(P0.05)。结论:口服淫羊藿苷元微乳制剂能有效治疗小鼠ITP模型血小板减少症状,其作用机制可能与激动C-Mpl受体刺激血小板增殖分化有关。
[Abstract]:Aim: to study the therapeutic effect of icariin on immunologic thrombocytopenic purpura (ITP) model of mice with immune thrombocytopenic purpura. Methods: male BALB/c mice were randomly divided into 5 groups. Except for normal control group, mice were induced by intraperitoneal injection of guinea pig antiplatelet serum (1: 4100 渭 L) every 1 day from day 1 to day 9. The platelets of isolated BALB/c mice were injected subcutaneously into guinea pigs for 5 weeks. The guinea pig anti-platelet serum .4 group ITP model mice were given intragastric administration for 10 days: microemulsion matrix. Recombinant human thrombopoietinine rh TPO)3 1000U kg-1, icariin microemulsion Y003: 1.25 and 5.0 mg / kg -1, control group were given microemulsion matrix 路d 11 to kill the animals, blood, femur, spleen were collected, and the following indexes were measured: blood routine test, visceral body ratio, The level of thrombopoietin (TPO) in serum and the pathology of spleen were determined by flow reticulated platelet count Elisa. Results compared with the model group, there were significant differences in peripheral platelet count, reticulocyte ratio, visceral body ratio and serum TPO content between the control group and the model group. Conclusion: Icariin microemulsion can effectively treat thrombocytopenia in ITP model mice, and its mechanism may be related to the activation of C-Mpl receptor to stimulate platelet proliferation and differentiation.
【作者单位】：北京大学药学院分子与细胞药理学系;鲁南制药集团股份有限公司新药药理中心;北京大学天然药物与仿生药物国家重点实验室;
【基金】：山东省自然科学基金资助项目(ZR2015QL002)
【分类号】：R285.5;R-332

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