前列腺素E1对小鼠子宫巨噬细胞表型和功能活性的影响

发布时间：2018-05-07 16:02

本文选题：前列腺素E_1 + 巨噬细胞　；参考：《河南师范大学》2011年硕士论文

【摘要】：目的:通过观察前列腺素E_1对正常小鼠子宫巨噬细胞表型和功能活性的影响,探讨其免疫抑制机理以及与母胎免疫耐受之间的关系。方法:正常昆明种小鼠随机分为正常对照组(DZ)和三个实验组:低浓度组(D组),中浓度组(Z组),高浓度组(G组),实验组分别尾静脉注射1303ng/kg/d、2606ng/kg/d、3909ng/kg/d的前列腺素E_1(将前列腺素E_1溶于0.2ml生理盐水),相应对照组分别用灭菌生理盐水(0.2ml)处理,连续注射5d,分别于末次注射后1h、3h、6h、12h、36h取子宫,制冰冻切片与石蜡切片,并用非特异性酯酶染色与免疫组织化学方法检测子宫巨噬细胞活性及其功能活性的变化。结果:(1)与对照组相比,各个实验组注射前列腺素1h后子宫内膜、子宫肌层和子宫外膜CD14~+巨噬细胞明显减少(P0.01),CD14~+表达下降。注射PGE_13h后,各浓度实验组子宫内膜、子宫外膜巨噬细胞均极显著减少,CD14~+表达量降低,子宫肌层CD14~+巨噬细胞数极显著性增加(P0.01),CD14~+表达量增加。注射PGE_16h后,各浓度实验组子宫内膜巨噬细胞均极显著减少(P0.01),CD14~+表达量下调,各浓度实验组子宫外膜巨噬细胞显著减少(P0.05),CD14~+表达量下调(2)对照组小鼠子宫未检测到CD204~+的表达,而相应实验组只在中浓度组的12h与36h组与高浓度组的6h、12h与36h组检测到CD204~+的表达,且表达趋势与CD14~+相似。高浓度组较中、低浓度组在36h极显著性增加(P0.01)。(3)与对照组相比,各个实验组注射前列腺素1h后子宫内膜α-NAE+巨噬细胞均显著减少,子宫外膜α-NAE+巨噬细胞均显著增加。注射PGE_112h后,各实验组的子宫内膜巨噬细胞均显著增加,子宫肌层巨噬细胞则极显著性减少。注射PGE_136h后,高浓度实验组子宫内膜、子宫肌层、子宫外膜巨噬细胞均极显著增加。结论:(1)PGE_1可以抑制子宫巨噬细胞功能活性,促进其从子宫内膜向子宫外膜迁移,这种效应与其半衰期与剂量呈相关性。(2)一定浓度的PGE_1能够促进子宫巨噬细胞膜受体CD204~+的表达。(3)PGE_1通过调节子宫巨噬细胞的数量以及膜受体CD14~+、CD204~+的表达,从而参与妊娠期母胎免疫耐受的发生和维持。
[Abstract]:Aim: to investigate the effect of prostaglandin E _ 1 on the phenotype and functional activity of normal mouse uterine macrophages, and to explore the mechanism of its immunosuppression and the relationship between prostaglandin E _ 1 and maternal and fetal immune tolerance. Methods: normal Kunming mice were randomly divided into normal control group (DZ) and three experimental groups: low concentration group (D group), middle concentration group (group Z), high concentration group (group G) and caudal injection of prostaglandin E1 (prostaglandin E 1) of 1303 ng / kg / d 2606ng / kg / d respectively. The corresponding control group was treated with sterilized saline (0.2 ml), while the control group was treated with sterilized saline (0.2 ml). After 5 days of continuous injection, the uterus was collected at 1 h, 3 h, 6 h, 12 h and 36 h after the last injection. Frozen sections and paraffin sections were prepared. The changes of the activity and function of uterine macrophages were detected by non-specific esterase staining and immunohistochemical method. Results compared with the control group, the expression of CD14 ~ macrophage in myometrium and adventitia decreased significantly in each experimental group after 1 h of prostaglandin injection. After the injection of PGE_13h, the expression of CD14 ~ in the outer membrane macrophages was significantly decreased, and the number of CD14 ~ macrophages in the myometrium was significantly increased in each concentration group, and the expression of CD14 ~ was significantly increased in the myometrium of the uterus. After injection of PGE_16h, the expression of CD14 ~ in endometrial macrophages of each concentration group was significantly decreased, while that of adventitia macrophages in each concentration group was significantly decreased (2) the expression of CD204~ was not detected in the uterus of mice in the control group. The expression of CD204~ in the corresponding experimental group was detected only at 12h and 36h in the middle concentration group and 6h and 36h in the high concentration group, and the expression trend was similar to that in the CD14h group. Compared with the control group, the 伪 -NAE macrophages and 伪 -NAE macrophages in the endometrium of each experimental group were significantly decreased and the 伪 -NAE macrophages in the outer membrane of uterus were significantly increased after 1 h of prostaglandin injection. After PGE_112h injection, the endometrial macrophages in each experimental group increased significantly, while the myometrium macrophages decreased significantly. After PGE_136h injection, the number of endometrial, myometrium and adventitia macrophages in the experimental group increased significantly. Conclusion PGE1 can inhibit the functional activity of macrophages and promote the migration of macrophages from endometrium to the outer membrane of uterus. This effect was related to its half-life and dose. (2) PGE_1 at a certain concentration could promote the expression of CD204~ on the membrane receptor of uterine macrophage. PGE1 could regulate the number of macrophages and the expression of CD14 ~ + CD204~ in uterine macrophage. So as to participate in the development and maintenance of maternal and fetal immune tolerance during pregnancy.
【学位授予单位】：河南师范大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

【参考文献】