睡眠剥夺大鼠腹外侧视前区腺苷水平的变化

发布时间：2018-05-07 22:42

本文选题：腹外侧视前区 + 腺苷　；参考：《安徽医科大学学报》2012年07期

【摘要】：目的研究睡眠剥夺大鼠促睡眠中枢腹外侧视前区(VLPO)内腺苷变化规律。方法将SD大鼠随机分为对照组和睡眠剥夺组。采用脑立体定位、微透析、多导睡眠描计技术和高效液相色谱法观察两组大鼠的睡眠剥夺前、睡眠剥夺期以及睡眠剥夺后恢复期VLPO内腺苷含量变化。结果与对照组比较,睡眠剥夺组睡眠剥夺前觉醒(W)、非快速眼动(NREM)睡眠和快速眼动(REM)睡眠无明显变化;而在睡眠剥夺期W时间增加58.5%(P0.01),NREM时间减少55.7%(P0.01),REM时间差异无统计学意义;在睡眠剥夺后恢复期W时间减少18.4%(P0.05),NREM时间增加17.4%(P0.05),REM时间差异无统计学意义。与对照组比较,睡眠剥夺开始的2 h内睡眠剥夺组的VLPO内腺苷相对含量无显著变化。而随后的4 h VLPO内腺苷相对含量明显升高(P0.05)。睡眠剥夺后恢复期的3 h内睡眠剥夺组的VLPO内腺苷相对含量显著减少(P0.05)。结论腺苷在VLPO内参与睡眠-觉醒周期的调节。
[Abstract]:Objective to study the changes of adenosine in the ventrolateral preoptic area (VLPO) of sleep promoting center in sleep deprived rats. Methods SD rats were randomly divided into control group and sleep deprivation group. The changes of adenosine content in VLPO before, during and after sleep deprivation were observed by brain stereotaxy, microdialysis, polysomnography and high performance liquid chromatography. Results compared with the control group, there were no significant changes in the sleep before sleep deprivation, NREM (non-rapid eye movement) sleep and REM) sleep in the sleep deprivation group, but there was no significant difference in the REM time between the sleep deprivation group and the control group when the W time was increased by 58.5% (P 0.01) and the NREM time was decreased by 55.7% (P 0.01). There was no significant difference in the time of NREM between P 0.05 and P 0.05 after sleep deprivation, and there was no significant difference in the time of NREM between P 0.05 and P 0.05 after sleep deprivation. Compared with the control group, the relative content of adenosine in VLPO in the sleep deprivation group had no significant change within 2 hours after sleep deprivation. However, the relative content of adenosine in VLPO increased significantly after 4 h. The relative content of adenosine in VLPO of sleep deprivation group decreased significantly within 3 hours after sleep deprivation. Conclusion adenosine is involved in the regulation of sleep-arousal cycle in VLPO.
【作者单位】：安徽医科大学基础医学院生理学教研室;安徽中医学院生理学教研室;
【基金】：国家自然科学基金(编号:81071075) 教育部博士点基金(编号:20093420120004) 安徽省高校优秀青年人才基金(编号:2009SQRZ047)
【分类号】：R741

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