改善Ⅱ型糖尿病大鼠糖代谢运动模型的建立

发布时间：2018-05-09 05:31

本文选题：游泳运动 + Ⅱ型糖尿病　；参考：《中国实验动物学报》2017年03期

【摘要】：目的建立运动改善Ⅱ型糖尿病大鼠糖代谢模型,为Ⅱ型糖尿病运动处方的建立提供理论参考。方法 SPF级雄性8周龄Wistar大鼠45只,随机抽取32只在高糖高脂饲料喂养7周的基础上腹腔注射小剂量链脲佐菌素(STZ)缓冲液,建立Ⅱ型糖尿病大鼠模型。将正常大鼠和造模成功的大鼠分为4组:空白对照组(C组)、单纯运动组(CE组)、糖尿病对照组(DM组)、糖尿病运动组(DME组)。运动组采用改进的Ploug训练方案,60min/d,每周训练6 d,共训练8周。分别在高脂饲料喂养7周后尾静脉取血测定空腹血糖和血清胰岛素,造模后基线时间和运动8周末尾静脉取血测定空腹血糖(FBG),运动8周末眶后取血测定血清胰岛素(FINS),并计算胰岛素抵抗指数(HOMA-IR)。结果 (1)7周高糖高脂喂养后,与正常组相比,高脂组FBG、FINS和HOMA-IR含量显著升高。(2)8周运动干预后,DM组和DME组FINS水平显著低于C组和CE组,FBG和HOMA-IR显著高于C组和CE组;DME组FINS水平显著高于DM组,FBG和HOMA-IR显著低于DM组。DM组和DME组体重显著低于C组和CE组;DME组与DM组、CE组与C组组间体重差异无显著性。结论 (1)7周高糖高脂喂养联合一次性腹腔注射STZ(30 mg/kg)成功建立Ⅱ型糖尿病大鼠模型;(2)60 min/d共8周的游泳运动模式能够改善Ⅱ型糖尿病大鼠糖代谢,是研究运动锻炼预防和改善糖尿病机理的理想动物模型。
[Abstract]:Objective to establish a model of glycometabolism in type II diabetic rats and to provide a theoretical reference for the establishment of type II diabetic exercise prescription. Methods 45 rats aged 8 weeks of age SPF were randomly selected and 32 rats were randomly selected for 7 weeks of high sugar and high fat feed for 7 weeks and intraperitoneally injected with small dose of streptozotocin (STZ) buffer to establish type II diabetes mellitus. Rat model. The rats were divided into 4 groups: blank control group (group C), simple exercise group (group CE), diabetes control group (group DM), diabetes exercise group (group DME). The exercise group adopted an improved Ploug training program, 60min/d, training 6 D per week for 8 weeks. After feeding high fat feed for 7 weeks, the tail vein blood was taken to determine empty stomach, respectively. Blood glucose and serum insulin, blood glucose (FBG) was measured at baseline and 8 weekend after exercise, and serum insulin (FINS) was measured after 8 weeks of exercise, and insulin resistance index (HOMA-IR) was calculated. Results (1) after 7 weeks high glucose and high fat feeding, the content of FBG, FINS and HOMA-IR in high fat group increased significantly. (2) 8 The level of FINS in group DM and DME group was significantly lower than that of group C and group CE, and FBG and HOMA-IR were significantly higher than that of group C and CE group, and FINS level in DME group was significantly higher than that in DM group. Lipid feeding combined with a one-time intraperitoneal injection of STZ (30 mg/kg) successfully established the model of type II diabetic rats. (2) the swimming exercise pattern of 60 min/d for 8 weeks can improve the glucose metabolism in type 2 diabetic rats. It is an ideal animal model for the study of exercise prevention and improvement of the mechanism of diabetes.

【作者单位】：北京体育大学运动人体科学学院;成都大学体育学院;成都体育学院;
【基金】：四川省教育厅人文社会科学重点研究基地—四川休闲体育产业发展研究中心课题资助(编号:XXTYCY2015C03) 成都大学校青年自然科学基金资助(编号:2017XJZ16)
【分类号】：R-332;R587.1

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