腺苷后处理对大鼠心肌缺血再灌注损伤后HSP70表达及心肌细胞凋亡的影响

发布时间：2018-05-09 05:57

本文选题：腺苷 + 心肌缺血再灌注损伤　；参考：《中西医结合心脑血管病杂志》2012年04期

【摘要】：目的通过观察腺苷后处理对大鼠心肌缺血再灌注损伤后热休克蛋白70(HSP70)表达及凋亡的影响,探讨腺苷后处理对心肌缺血再灌注损伤的保护机制。方法 24只成年雄性Wistar大鼠随机分为假手术对照组(Sham组)、缺血再灌注组(I/R组)、腺苷后处理组(AD组),采用结扎大鼠左冠状动脉前降支法制备大鼠心肌缺血再灌注模型;免疫组化SABC法检测HSP70表达;采用DNA原位末端缺口标记法(TUNEL)检测心肌细胞凋亡。结果与假手术组比较,I/R组与AD组HSP70表达水平均升高(P0.05),且AD组明显高于I/R组(P0.05)。心肌细胞凋亡率,I/R组与AD组较假手术组增高(P0.05),且AD组明显低于I/R组(P0.05)。结论腺苷后处理可增加心肌缺血再灌注损伤大鼠HSP70的表达,减轻心肌细胞凋亡,有效保护心肌缺血再灌注损伤。
[Abstract]:Objective to investigate the protective mechanism of adenosine postconditioning on myocardial ischemia-reperfusion injury by observing the expression and apoptosis of heat shock protein 70 (HSP70) after myocardial ischemia reperfusion injury in rats. Methods Twenty-four adult male Wistar rats were randomly divided into sham-operated control group, ischemia-reperfusion group and adenosine posttreatment group. Myocardial ischemia-reperfusion model was established by ligating left anterior descending coronary artery in rats. Immunohistochemical SABC method was used to detect the expression of HSP70 and DNA in situ Nick end labeling (Tunel) was used to detect the apoptosis of cardiomyocytes. Results compared with the sham operation group, the expression of HSP70 in the I / R group and AD group was significantly higher than that in the I / R group, and the expression level of HSP70 in AD group was significantly higher than that in the I / R group. The apoptosis rate of cardiac myocytes in group I / R and AD was significantly higher than that in group I / R, and was significantly lower in group AD than that in group I / R. Conclusion after adenosine treatment can increase the expression of HSP70 in myocardial ischemia-reperfusion injury rats, reduce myocardial cell apoptosis, and protect myocardial ischemia-reperfusion injury effectively.
【作者单位】：山西医科大学;山西医科大学第一医院;
【分类号】：R541.4

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