人乳头瘤病毒16型E7蛋白的生物信息学分析

发布时间：2018-05-09 13:29

本文选题：人乳头瘤病毒 + E蛋白　；参考：《中国病原生物学杂志》2017年08期

【摘要】：目的应用生物信息学预测分析人乳头瘤16型病毒(human papillomavirus type 16,HPV16)E7蛋白的结构特征与抗原表位。方法自NCBI GENE BANK获取HPV16E7蛋白的基因信息,应用ProtParam分析其编码的E7蛋白理化性质和一级结构;利用SOPMA分析E7蛋白的二级结构;应用SWISS MODEL的Automated mode预测E7蛋白的三维空间结构;使用NetNGlyc 1.0Server、NetPhos3.1Server、MotifyScan、SignalP及TMHMM预测蛋白质的翻译后修饰位点、信号肽切割位点和跨膜区;利用CLUSTALX将HPV16E7蛋白同其他高危型HPV E7蛋白进行同源性对比;应用ABCpred、BepiPred、IEDB、SYFPEITHI和NetMHCIIpan 3.1Server预测E7蛋白的优势抗原表位。结果生物信息学预测E7蛋白无规卷曲占52.04%,结构较松散,为不稳定性蛋白。该蛋白存在多个磷酸化位点。利用序列匹配度为46%的HPV45E7蛋白为模板得到同型二聚体三级预测结构。E7蛋白高度保守,且具有潜在的B细胞抗原表位1-8、15-22、33-46,CD8+T细胞抗原表位82-90、11-19、7-11、85-93、13-21和CD4+T细胞抗原表位9-23、73-87、10-24、7-21、21-35。结论 HPV16E7蛋白为不稳定蛋白。该蛋白高度保守,且具有潜在的B、T细胞抗原表位。
[Abstract]:Objective to predict and analyze the structure and epitope of HPV16 E7 protein of human papillomavirus type 16 papillomavirus type 16 by bioinformatics. Methods the gene information of HPV16E7 protein was obtained from NCBI GENE BANK, the physicochemical properties and primary structure of E7 protein were analyzed by ProtParam, the secondary structure of E7 protein was analyzed by SOPMA, the three-dimensional structure of E7 protein was predicted by Automated mode of SWISS MODEL. Using NetNGlyc 1.0 Server, NetPhos3.1 Server motif y ScanSignalP and TMHMM to predict post-translational modification sites, signal peptide cleavage sites and transmembrane regions, CLUSTALX was used to compare HPV16E7 protein homology with other high-risk HPV E7 proteins, and ABCpredNetNGlyc BepiPredIEDBSYFPEITHI and NetMHCIIpan 3.1Server were used to predict the dominant epitope of E7 protein. Results the random coil of E7 protein in bioinformatics was 52.04% and its structure was looser and it was an unstable protein. There are many phosphorylation sites in this protein. Using 46% HPV45E7 protein as template, the homotypic dimer tertiary predictive structure. E7 protein was highly conserved, and had potential B cell epitope 1-82233-46-CD8 T cell epitope 82-90 11-197-1175-95-9313-21 and CD4 T cell epitope 9-2373-83-83-81-2121-3535. Conclusion HPV16E7 protein is unstable protein. The protein is highly conserved and has a potential epitope of BU T cell antigen.
【作者单位】：中国医科大学临床三系;中国医科大学基础医学院病原生物学教研室;
【基金】：国家自然科学基金项目(No.81472439,81101989)
【分类号】：R373

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