人胎盘胎儿侧间充质干细胞无血清培养上清的抗氧化活性分析

发布时间：2018-05-12 11:32

本文选题：干细胞 + 组织工程　；参考：《中国组织工程研究》2017年05期

【摘要】：背景:目前关于间充质干细胞的研究大多集中于其免疫调节功能,而关于细胞和培养上清的其抗氧化能力的研究较为少见。目的:观察人胎盘胎儿侧间充质干细胞在无血清培养条件下,其上清的抗氧化能力。方法:采用无血清培养基培养胎儿侧胎盘间充质干细胞,分别于48 h收集P2-P6代细胞培养上清。在空白培养基中添加维生素C 100μmol/L作为阳性对照。检测P2-P6代人胎盘胎儿侧间充质干细胞培养上清的总抗氧化能力、清除活性氧自由基的能力和抗氧化酶活性。结果与结论:(1)抗氧化能力:各代次人胎盘胎儿侧间充质干细胞培养上清具有一定的抗氧化能力,且不同代次间的上清具有一定的差异,其中总抗氧化能力与40-80μmol/L的维生素C相当,各代次上清均具有一定的清除二苯基苦基苯肼自由基、羟自由基、超氧阴离子自由基等自由基清除能力;(2)抗氧化酶活性分析:各代次间的人胎盘胎儿侧间充质干细胞培养上清中均可以检测到一定水平的超氧化物歧化酶和谷胱甘肽过氧化物酶活性;(3)结果提示,在无血清条件下培养胎儿侧胎盘间充质干细胞的上清具有一定的抗氧化能力和抗氧化酶的活性。人胎盘胎儿侧间充质干细胞的抗氧化能力与其旁分泌机制有关,但其中的抗氧化成分及分子机制有待进一步的研究。
[Abstract]:Background: at present, most studies on mesenchymal stem cells focus on their immunomodulatory function, while the studies on the antioxidant capacity of cells and culture supernatants are rare. Aim: to observe the antioxidation ability of human placental fetal lateral mesenchymal stem cells (HPMSCs) in serum-free culture. Methods: fetal placental mesenchymal stem cells were cultured in serum-free medium and the supernatants of P2-P6 cells were collected at 48 h. Vitamin C 100 渭 mol/L was added to the blank medium as positive control. The total antioxidant capacity, scavenging capacity and antioxidant enzyme activity of the supernatant of human fetal placenta lateral mesenchymal stem cells cultured with P2-P6 were measured. Results and conclusion: the supernatant of human fetal lateral mesenchymal stem cells cultured in different generations had a certain antioxidant capacity, and the supernatant of different generations had some differences, and the total antioxidant capacity was similar to that of vitamin C of 40-80 渭 mol/L. The supernatant of each generation had a certain scavenging effect on diphenyl-picrylphenylhydrazine free radical and hydroxyl radical. Analysis of antioxidant enzyme activity: superoxide dismutase and glutathione can be detected in the supernatant of human placenta fetal lateral mesenchymal stem cells culture from different generations. The results of peptide peroxidase activity suggested that, The supernatant of fetal placental mesenchymal stem cells cultured in serum-free medium had a certain antioxidant activity and antioxidant enzyme activity. The antioxidation ability of human placental fetal lateral mesenchymal stem cells is related to its paracrine mechanism, but the antioxidant components and molecular mechanisms need to be further studied.
【作者单位】：宁夏医科大学临床医学院;宁夏医科大学总医院人类干细胞研究所;
【基金】：国家自然科学基金(81460247)~~
【分类号】：R329.2

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