γ-干扰素及外源性吲哚对泌尿生殖道沙眼衣原体生长影响的探讨

发布时间：2018-05-14 08:19

本文选题：沙眼衣原体 + Hela细胞　；参考：《中国人兽共患病学报》2017年02期

【摘要】：目的探讨外源性吲哚及γ-干扰素对国内沙眼衣原体优势株E-UW-5/Cx型标准株及临床株生长的影响,并与国外优势株D-UW-5/Cx进行比较。方法分别用DMEM-10、DMEM-10加5ng/mL重组人γ-干扰素和DMEM-10加5ng/mL重组人γ-干扰素及50μmol外源性吲哚三种细胞培养液培养沙眼衣原体至48h,甲醇固定后计数包涵体,观察γ-干扰素及外源性吲哚对E型、D型沙眼衣原体标准株及临床株生长的影响。结果 DMEM-10+IFN组衣原体包涵体计数明显低于DMEM-10组和DMEM-10+IFN+IND组,差异有统计学意义,P0.05;DMEM-10组和DMEM-10+IFN+IND组包涵体计数差异无统计学意义;E型标准株、临床株与D型标准株、临床株之间差异无统计学意义,P0.05。结论在IFN-γ作用下,沙眼衣原体的生长繁殖受到明显抑制,加入外源性吲哚后,泌尿生殖道沙眼衣原体可以逃逸IFN-γ介导的清除作用,恢复其感染活力。
[Abstract]:Objective to investigate the effects of exogenous indoles and interferon 纬 on the growth of E-UW-5/Cx type standard strain and clinical strain of Chlamydia trachomatis dominant strain in China, and compare them with that of foreign dominant strain D-UW-5/Cx. Methods Chlamydia trachomatis was cultured for 48 h with DMEM-10 DMEM-10 plus 5ng/mL recombinant human interferon- 纬 and DMEM-10 with 5ng/mL recombinant human interferon-gamma and 50 渭 mol exogenous indoles respectively. The inclusion bodies were counted after methanol fixation. The effects of interferon 纬 and exogenous indole on the growth of standard and clinical strains of Chlamydia trachomatis E type D were observed. Results the inclusion body count of chlamydia in DMEM-10 IFN group was significantly lower than that in DMEM-10 group and DMEM-10 IFN IND group. There was no significant difference in inclusion body count between DMEM-10 group and DMEM-10 IFN IND group. There was no significant difference between clinical strains (P 0.05). Conclusion the growth and reproduction of chlamydia trachomatis were obviously inhibited by IFN- 纬. After exogenous indole, chlamydia trachomatis in genitourinary tract could escape IFN- 纬 mediated clearance and recover its infection activity.
【作者单位】：泰山医学院附属医院;天津医科大学总医院;
【基金】：山东省自然科学基金项目(No.ZR2011HL009) 泰安市科技发展计划项目(No.2016NS1167)联合资助~~
【分类号】：R374

【相似文献】