六味地黄丸对椎间盘体外退变模型椎间盘细胞外基质组分的影响

发布时间：2018-05-14 08:57

  本文选题：椎间盘退化 + 肿瘤坏死因子α　； 参考：《中国组织工程研究》2017年16期

【摘要】：背景:六味地黄丸作为补益肝肾名方,临床应用其治疗腰痛已取得良好的效果,而有关六味地黄丸治疗肾虚型腰痛的作用机制尚不明确。目的:观察六味地黄丸对兔椎间盘体外退变模型细胞外基质组分的影响,探讨六味地黄丸防治椎间盘退变的疗效。方法:将20只新西兰兔带终板的L1-L6椎间盘100个随机分为空白对照组、肿瘤坏死因子α组、p38-JNK/SAPK阻断组、六味地黄丸组、肿瘤坏死因子α+六味地黄丸组,每组20个。肿瘤坏死因子α组培养液中含10 mg/L肿瘤坏死因子α2μL,p38-JNK/SAPK阻断组培养液中含p38MAPK特异性阻断剂SB203580,JNK特异性阻断剂SP600125各20μmol/L 10μL,六味地黄丸组培养液中含体积分数为10%六味地黄丸血清,肿瘤坏死因子α+六味地黄丸组含10 mg/L肿瘤坏死因子α2μL和体积分数为10%六味地黄丸血清,分别于培养的第2,4,8,14天收集标本待测。结果与结论:在培养基中加入肿瘤坏死因子α能明显上调Ⅰ型胶原mRNA和蛋白的表达,下调糖胺多糖含量、硫酸软骨素/硫酸角质素比值、透明质酸含量,蛋白多糖mRNA和蛋白的表达、Ⅱ型胶原mRNA和蛋白的表达(P0.05),六味地黄丸含药血清可部分对抗肿瘤坏死因子α所导致的改变,提示六味地黄丸可在一定程度上延缓了椎间盘退变。
[Abstract]:Background: as a famous prescription for tonifying liver and kidney, Liuwei Dihuang Pill has achieved good results in the treatment of low back pain, but the mechanism of Liuwei Dihuang Pill in treating low back pain of kidney deficiency type is not clear. Aim: to observe the effect of Liuwei Dihuang pill on extracellular matrix components of rabbit intervertebral disc degeneration model in vitro and to explore the effect of Liuwei Dihuang pill on the prevention and treatment of disc degeneration. Methods: 100 L1-L6 intervertebral discs with endplate in 20 New Zealand rabbits were randomly divided into control group (n = 20), tumor necrosis factor 伪 group (n = 20), and tumor necrosis factor 伪 group (n = 20). TNF- 伪 group contained 10 mg/L TNF- 伪 2 渭 L p38-JNK-SAPK blocking medium containing 10 渭 mol/L 10 渭 L p38MAPK specific blocker SB203580 and 10 渭 mol/L 10 渭 L SP600125, and the volume fraction of 10% Liuwei Dihuang pills was 10% Liuwei Dihuang Pill. Tumor necrosis factor 伪 Liuwei Dihuang pill group contained 10 mg/L tumor necrosis factor 伪 2 渭 L and volume fraction of 10% Liuwei Dihuang pill serum. Results and conclusion: tumor necrosis factor 伪 can significantly up-regulate the expression of type I collagen mRNA and protein, down-regulate the content of glycosaminoglycan, the ratio of chondroitin sulfate to keratin sulfate, and the content of hyaluronic acid. The expression of proteoglycan mRNA and protein, the expression of type 鈪，

本文编号：1887203