新型灌胃器经胃给药法在新生SD大鼠中的应用及效果评价

发布时间：2018-05-15 15:24

本文选题：新生大鼠 + 灌胃　；参考：《广东医学》2017年03期

【摘要】：目的制作一种简单、方便,适合新生大鼠灌胃给药的灌胃器。方法选取141只新生大鼠,按随机数表法分4组,即对照组与低、中、高剂量组。采用新型灌胃器于生后48 h内对每只新生大鼠灌予生理盐水或二十二碳六烯酸(DHA)600、300、100 mg/kg,1次/d,0.01 m L/(g·次),共灌喂21 d,观察不同剂量药物对新生鼠的存活情况、体质量变化及神经发育的影响。结果 128只新生鼠存活,动物存活率90.78%。新生鼠出生体重(7.37±0.063)g。7日龄体重:对照组与低、中、高剂量组分别为(18.57±0.76)、(1.66±0.89)、(14.23±0.49)、(16.13±1.02)g;14日龄体质量分别为(37.60±1.32)、(36.24±0.84)、(33.63±1.04)、(31.69±1.77)g;21日龄体质量分别为(53.01±11.75)、(58.52±6.62)、(52.47±8.18)、(45.90±13.17)g。顺利完成实验。结论新型灌胃器对新生大鼠的口腔、食管黏膜的损伤小,给药依从性好、给药时间快速,新生大鼠存活率高,适合用于对大批量的新生大鼠进行长期、有效的灌胃,有利于顺利完成实验过程,为通过新生大鼠进行药物、营养的相关研究提供了建制新生大鼠动物模型的良好的方法与依据。
[Abstract]:Objective to make a simple, convenient and suitable gavage apparatus for the fresh-born rats. Methods 141 newborn rats were selected and divided into 4 groups according to the random number table method. The control group and the low, middle and high dose group were given a new type of intragastric irrigator in 48 h after birth to each newborn rat with physiological salt water or twenty-two carbon six enoic acid (DHA) 600300100 mg/kg, 1 times /d, 0 .01 m L/ (G.) was fed a total of 21 d to observe the survival of the newborn rats, the changes of body mass and the effect of nerve development. Results 128 newborn rats survived, the survival rate of the newborn rats was 90.78%. birth weight (7.37 + 0.063) G.7 day old weight: the control group was (18.57 + 0.76) and (1.66 + 0.89) in the low, middle and high dose group, (14.23. 0.89), 14.23. (16.13 + 1.02) g, 14 days old body mass were (37.60 + 1.32), (36.24 + 0.84), (33.63 + 1.04) and (31.69 + 1.77), respectively. The mass of 21 days age body was respectively (0.84 + 37.60), and g. successfully completed the experiment. Conclusion the new type of gavage apparatus has little damage to the oral mucosa of newborn rats, and the compliance of the administration is good. The time of drug delivery is rapid and the survival rate of newborn rats is high. It is suitable for the long-term and effective gastric perfusion of large batch of neonatal rats, which is beneficial to the successful completion of the experimental process. The related research on nutrition provides a good method and basis for the establishment of the model of the new rat.

【作者单位】：广州医科大学附属第三医院儿科;
【基金】：广州市科技和信息化局科研课题(编号:2014Y2-00164) 广州医科大学青年科研资助项目(编号:2013A26)
【分类号】：R722.1;R-332

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