五指山小型猪、西藏小型猪和巴马小型猪对外源性脂肪的敏感性观察

发布时间：2018-05-15 16:11

本文选题：小型猪 + 血脂代谢　；参考：《中国比较医学杂志》2017年06期

【摘要】：目的观察五指山小型猪、西藏小型猪和巴马小型猪对外源性脂肪的敏感性。方法取雄性五指山小型猪、西藏小型猪和巴马小型猪各5只,分别采用静脉注射脂肪乳和饲喂高脂饲料,分别检测静脉注射脂肪乳后0.5、1、1.5、2、2.5、3 h和饲喂高脂饲料后12、24、36、48、60、72、84、120 h的血清胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(LDL-C)和甘油三酯(TG)水平,并计算曲线下面积变化ΔAUC值。结果注射脂肪乳后3种小型猪甘油三酯(TG)均明显升高(P0.01),在注射后0.5 h达到峰值,其升高程度和ΔAUC值高低依次为西藏小型猪五指山小型猪巴马小型猪,而TC、LDL-C和HDL-C则无明显变化(P0.05);高脂饲料饲喂饮食诱导后3种小型猪TC、LDL-C、HDL-C和TG均明显升高(P0.05,P0.01),其中,五指山小型猪TC、LDL-C在饲喂后36 h、HDL-C在饲喂后48 h、TG在饲喂后24 h达到峰值,巴马小型猪和西藏小型猪TC、LDL-C、HDL-C在饲喂后48-60 h、TG在饲喂后36 h达到峰值,TC、LDL-C、HDL-C的ΔAUC值高低依次为五指山小型猪巴马小型猪西藏小型猪。结论静脉注射脂肪乳3种小型猪对TG敏感,脂耐量值高低依次为西藏小型猪五指山小型猪巴马小型猪;高脂饲料饲喂对3种小型猪TC、LDL-C、HDL-C敏感,脂耐量值高低依次为五指山小型猪巴马小型猪西藏小型猪。
[Abstract]:Objective to observe the sensitivity of Wuzhishan miniature pig, Tibet miniature pig and Bama miniature pig to exogenous fat. Methods five male Wuzhishan mini-pigs, five Tibetan mini-pigs and five Bama mini-pigs were fed with fat milk and high-fat feed respectively. The levels of serum cholesterol, low density lipoprotein (LDL-C), high density lipoprotein (LDL-C), triglyceride (TG) and triglyceride (TGG) were measured after intravenous injection of adipose milk at 1. 5 ~ 1. 5 and 2. 5 h after feeding with high fat diet, and the area change 螖 AUC value under the curve was calculated, and the changes of serum cholesterol, low density lipoprotein (LDL-C), high density lipoprotein (HDL-C) and triglyceride (TG) under the curve were calculated. Results the triglyceride (TG) of the three mini-pigs increased significantly after fat milk injection, and reached the peak at 0.5 h after injection. The elevation degree and 螖 AUC value of the three kinds of mini-pigs were in the order of Wuzhishan mini-pig, Bama mini-pig, Tibet mini-pig, and the peak value of 螖 AUC at 0.5 h after injection. However, there was no significant change in LDL-C and HDL-C, and HDL-C and TG in LDL-C were significantly increased in the three mini-pigs after fed with high-fat diet. Among them, TCU LDL-C of Wuzhishan mini-pig reached its peak at 48 h after feeding, and the levels of HDL-C and TG were significantly increased at 24 h after feeding. The 螖 AUC values of HDL-C of Bama mini-pigs and Tibetan mini-pigs reached the peak at 36h after feeding for 48 to 60 hours. The order of 螖 AUC value was Wuzhishan mini-pig, Bama mini-pig, Tibet mini-pig. Conclusion three kinds of small pigs with intravenous fat milk are sensitive to TG, and the level of lipid tolerance is the same as that of Tibet mini-pig Wuzhishan mini-pig Bama mini-pig, and the high-fat feed is sensitive to three kinds of miniature pigs, TCU LDL-C and HDL-C. The level of lipid tolerance in turn was Wuzhishan mini-pig, Bama mini-pig, Tibet mini-pig.
【作者单位】：浙江中医药大学;
【基金】：浙江省科技厅实验动物科技计划项目(2012C37078) 浙江中医药大学比较医学创新团队(XTD201301)
【分类号】：R-332

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