趋化因子12促进少突胶质前体细胞的增殖

发布时间：2018-05-15 16:51

本文选题：CXCL + OPCs　；参考：《第三军医大学学报》2016年08期

【摘要】：目的体外研究趋化因子12(CXCL12)对大鼠少突胶质前体细胞(oligodendrocyte precursor cells,OPCs)增殖的影响及其机制。方法振荡分离和差速贴壁等方法获得OPCs,采用免疫细胞化学技术进行鉴定;CCK-8检测OPCs在不同浓度CXCL12(0、5、10、20 ng/m L)作用不同时间点(0、24、48、72 h)的增殖,Western blot检测OPCs在各浓度下72 h后下游CXCR4、CXCR7、MMP9蛋白表达;CXCR4 siRNA、CXCR7 siRNA分别干扰CXCR4、CXCR7蛋白表达后,检测在20 ng/m L CXCL12下OPCs在各时间点的增殖,Western blot检测该条件下CXCR4、CXCR7、MMP9蛋白表达;MMP9 siRNA干扰MMP9蛋白表达后,检测在20 ng/m L CXCL12下OPCs在各时间点的增殖。结果在一定浓度范围内,CXCL12能够明显促进OPCs增殖,并且促进OPCs内CXCR4、CXCR7、MMP9蛋白表达。与0 ng/m L相比,20 ng/m L CXCL12作用72 h后,OPCs增殖及CXCR4、CXCR7、MMP9蛋白表达增加最明显(P0.05,P0.01);CXCR4、CXCR7分别干扰后,OPCs增殖受到抑制,MMP9蛋白表达量也明显降低(P0.05,P0.01);MMP9干扰后,OPCs增殖受到抑制(P0.05)。结论 CXCL12对OPCs增殖有明显促进作用,与CXCR4、CXCR7、MMP9蛋白表达上调相关。
[Abstract]:Objective to investigate the effect of chemokine CXCL12 on the proliferation of oligodendrocyte precursor cells and its mechanism in rats. Methods OPC was obtained by oscillatory isolation and differential adherence. The proliferation of OPCs was detected by Western blot at different time points (CXCL12010, 10, 10, 20 ng/m L). The proliferation of OPCs was detected at 72 h after 72 h of different concentrations of CXCL12CK-8. CXCR4 siRNA-CXCR7 siRNA interferes with the expression of CXCR4CXCR7 protein. The proliferation of OPCs at different time points was detected at 20 ng/m L CXCL12. Western blot was used to detect the expression of CXCR4, CXCR7, MMP9, and OPCs at 20 ng/m L CXCL12. Results CXCL12 could significantly promote the proliferation of OPCs and the expression of CXCR7MMP9 protein in OPCs. Compared with 0 ng/m L, the proliferation of OPCs and the expression of CXCR4CXCR7MMP9 protein were significantly increased after treated with 20 ng/m L CXCL12 for 72 h, respectively. The expression of P0.05, P0.01 and CXCR4CXCR7 was significantly inhibited by P0.05P0.01MMP9 protein expression, and the proliferation of OPCs was inhibited by P0.05P0.01MMP9. Conclusion CXCL12 can significantly promote the proliferation of OPCs, which is related to the up-regulation of CXCR4, CXCR7, MMP9 protein.
【作者单位】：第三军医大学新桥医院骨科;
【基金】：国家自然科学基金面上项目(81471262)~~
【分类号】：R392.12

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