分子或离子与人血清白蛋白的相互作用以及荧光碳纳米颗粒的研究

发布时间：2018-05-17 17:18

本文选题：人血清白蛋白 + 孔雀石绿　；参考：《兰州大学》2012年硕士论文

【摘要】：人血清白蛋白(human serum albumin, HSA)是血浆中含量最多的一种蛋白质,和其它蛋白质相比,HSA具有分子量小、溶解性大、稳定性较好、很好的亲和性及能同多种物质结合(如金属离子、脂肪酸、氨基酸、激素及药物等)的特点。通过研究物质和HSA的相互作用,就可以得到这些物质在生物体内被转运、分配和代谢过程中的重要信息。因此,对金属离子、有机小分子与HSA相互作用的研究是很有意义的,该课题已经成为生命科学、化学及临床医学研究的重要课题之一。本论文除了研究分子或离子与HSA的相互作用之外,还对荧光碳纳米颗粒(CNPs)的性质进行了研究。由于碳是自然界中含量最丰富的元素之一,且碳材料的毒性都非常低,所以碳纳米材料一直是科技创新的前沿领域。本论文在前人研究的基础上,开展了以下三个方面的工作： 1.在模拟生理条件下,研究了孔雀石绿与HSA的相互作用。利用荧光光谱、圆二色谱和傅里叶变换红外光谱技术确定了它们之间相互作用的结合常数、主要作用力以及孔雀石绿对HSA二级结构的影响。利用分子模拟技术得到的热力学参数,确定了二者间的相互作用力。实验结果表明孔雀石绿可以使HSA发生荧光猝灭,猝灭机理为静态猝灭。它们之间的结合常数KSV=2.9×104Lmol-1,结合位点数n=1.2。 2.在模拟生理条件下,利用荧光光谱和紫外光谱的方法研究了金属离子Mo(Ⅵ)与HSA的相互作用。实验结果表明Mo(Ⅵ)可以使HSA发生荧光猝灭,猝灭机理为静态猝灭。它们之间的结合常数KSV=1.29×104Lmol-1,结合位点数n=1.01。 3.利用硝酸氧化煤油灰的方法制得CNPs,它具有很好的水溶性,在水溶液中可以稳定存在三个月以上且其荧光强度不会降低。通过分析CNPs的扫描电镜图可知CNPs的平均粒径约为50nm左右。为了提高它的性能,用硫脲(TC)对其表面进行了修饰,修饰后的CNPs (CNPs-TC)的荧光性质发生了一定的变化,而且CNPs-TC与CNPs的荧光强度对pH值变化的反应不同。此外,我们还研究了CNPs、CNPs-TC与金属离子的相互吸附作用。结果表明CNPs-TC对金属离子的吸附和沉降作用更为明显。
[Abstract]:Human serum albumin (HSA) is the most abundant protein in plasma. Compared with other proteins, HSA has the characteristics of small molecular weight, large solubility, good stability, good affinity and ability to combine with a variety of substances (such as metal ions, fatty acids, amino acids, hormones and drugs, etc.). Through the study of substances and HSA The interaction of these substances can be important information in the process of transport, distribution and metabolism of these substances in organisms. Therefore, it is very meaningful to study the interaction of metal ions and small organic molecules with HSA. The subject has become one of the important topics in the research of life science, chemistry and clinical medicine.
In addition to the study of the interaction of molecules or ions with HSA, the properties of fluorescent carbon nanoparticles (CNPs) are also studied. Since carbon is one of the most abundant elements in nature and the toxicity of carbon materials is very low, carbon nanomaterials have always been the frontiers of science and technology innovation.
On the basis of previous studies, this thesis has carried out the following three aspects:
1. the interaction between malachite green and HSA was studied under simulated physiological conditions. Using fluorescence spectroscopy, circular two chromatography and Fu Liye transform infrared spectroscopy, the interaction constants, main forces and the effect of malachite green on the structure of HSA two were determined. The interaction force between the two is determined. The experimental results show that the malachite green can cause the fluorescence quenching of HSA, and the quenching mechanism is static quenching. The binding constant between them is KSV=2.9 x 104Lmol-1, and the number of binding sites is n=1.2.
2. under simulated physiological conditions, the interaction between metal ions Mo (VI) and HSA was studied by fluorescence and UV spectra. The experimental results show that Mo (VI) can cause fluorescence quenching of HSA and the quenching mechanism is static quenching. The binding constant between them is KSV=1.29 * 104Lmol-1, and the number of bits n=1.01. is combined.
3. the CNPs is prepared by oxidation of kerosene ash with nitric acid. It has good water solubility. It can be stable in aqueous solution for more than three months and its fluorescence intensity will not decrease. By analyzing the scanning electron microscopy of CNPs, the average particle size of CNPs is about 50nm. In order to improve its performance, the surface of it is modified with thiourea (TC). The fluorescence properties of the modified CNPs (CNPs-TC) have changed a certain amount, and the reaction of the fluorescence intensity of CNPs-TC and CNPs to the change of pH value is different. In addition, we also studied the interaction between CNPs, CNPs-TC and metal ions. The results show that CNPs-TC has more obvious effect on the absorption and settlement of metal ions.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R341

【引证文献】