自发性糖尿病GK大鼠糖尿病周围神经病变模型的建立

发布时间：2018-05-19 16:08

本文选题：糖尿病神经病变 + 疾病模型　；参考：《天津医药》2016年08期

【摘要】：目的通过高脂饲养GK大鼠建立一种符合人类糖尿病周围神经病变(DPN)发病特点,又操作简单的DPN大鼠模型。方法 7~8周龄SPF级雄性自发性2型糖尿病GK大鼠30只,喂养高脂饲料造模。另取30只SPF级正常Wistar大鼠作为正常组,喂养普通饲料。每周监测动物血糖、体质量、饮水量、饲料量。分别于干预8周、12周、16周后检测血清糖化血红蛋白、坐骨神经传导速度,取对侧坐骨神经做HE染色,TUNEL染色计算细胞凋亡指数。结果随着造模时间延长,GK大鼠逐渐出现多饮、多食、生长迟缓等表现。与正常组相比,造模12周及16周的大鼠血糖、糖化血红蛋白升高(P0.01),感觉神经传导速度降低(P0.01),运动神经传导速度有一定下降趋势(12周P0.05,16周P0.05),坐骨神经病理形态及雪旺细胞凋亡情况均提示DPN的形成,并与正常大鼠形成鲜明对照(凋亡指数P0.01)。结论长期高脂饲料喂养的GK大鼠是DPN研究的良好模型,12周是较为成熟且经济的造模时间。
[Abstract]:Objective to establish a DPN rat model with high fat feeding in accordance with the characteristics of diabetic peripheral neuropathy (DPN). Methods Thirty SPF grade male GK rats with spontaneous type 2 diabetes were fed with high fat diet. Another 30 normal Wistar rats of SPF grade were taken as normal group and fed with normal diet. Blood sugar, body mass, drinking water and feed quantity were monitored weekly. Serum glycosylated hemoglobin and sciatic nerve conduction velocity were measured after 8 weeks and 12 weeks and 16 weeks, respectively. The contralateral sciatic nerves were taken for HE staining and Tunel staining to calculate apoptosis index. Results with the prolongation of model making time, GK rats gradually appeared more drink, more food and growth retardation. Compared with the normal group, the blood glucose levels of the rats at 12 and 16 weeks after modeling were compared with those in the control group. The HbA1c increased, the sensory nerve conduction velocity decreased, the motor nerve conduction velocity decreased, and the motor nerve conduction velocity decreased. The histopathological morphology of sciatic nerve and the apoptosis of Schwann cells indicated the formation of DPN. In contrast to normal rats, the apoptotic index (P0.01) was significantly different from that in normal rats. Conclusion GK rats fed with high fat diet for a long time are a good model for DPN study. 12 weeks is a more mature and economical time to model GK rats.
【作者单位】：成都中医药大学临床医学院;重庆市中医院内分泌科;成都中医药大学基础医学院;
【基金】：国家自然科学基金资助项目(81373783)
【分类号】：R587.2;R-332

【相似文献】