药对刺五加-栀子对慢性应激抑郁模型大鼠GSα-PKA-cAMP信号通路的影响

发布时间：2018-05-20 19:05

本文选题：药对 + 刺五加-栀子　；参考：《中国老年学杂志》2016年24期

【摘要】：目的探讨药对刺五加-栀子对慢性不可预见性应激模型大鼠GSα-PKA-c AMP信号通路的影响及其抗抑郁机制。方法健康雄性SD大鼠40只,随机分为空白组、模型组、阳性对照组、药对刺五加-栀子组,每组10只。采用慢性不可预见性应激方法建立抑郁模型。采用RT-PCR法检测GSα蛋白的表达;采用ELISA法检测环磷酸腺苷(c AMP)和蛋白激酶(PKA)的变化。结果给药4 w后,与空白组相比,模型组GSα蛋白表达和c AMP含量及PKA活性明显降低(P0.01);与模型组相比,阳性对照组上述指标表达增强(P0.01);与模型组相比,药对刺五加-栀子组上述指标表达增强(P0.01)。结论药对刺五加-栀子通过调节CUMS大鼠的海马、前额叶皮层中GSα、PKA、c AMP含量,从而调节GSα-PKA-c AMP信号通路发挥抗抑郁作用。
[Abstract]:Objective to investigate the effect of Acanthopanax senticosus (Acanthopanax senticosus)-Gardenia jasminoides on GS 伪-PKA-c AMP signaling pathway and its antidepressant mechanism in rats with chronic unpredictable stress. Methods Forty healthy male Sprague-Dawley rats were randomly divided into blank group, model group, positive control group and Acanthopanax senticosus Gardenia group with 10 rats in each group. The depression model was established by chronic unpredictable stress. The expression of GS 伪 protein was detected by RT-PCR method, and the changes of camp and PKA were detected by ELISA assay. Results after 4 weeks of administration, the expression of GS 伪 protein, the content of c AMP and the activity of PKA in the model group were significantly lower than those in the blank group, while the expression of the above indexes in the positive control group was enhanced compared with the model group, and the expression of the above indexes in the model group was higher than that in the model group, while that in the model group was higher than that in the model group. The expression of above indexes in Acanthopanax senticosus-Gardenia group was enhanced (P 0.01). Conclusion the antidepressant effect of Fructus Acanthopanax senticosus-Gardenia on GS 伪 -PKA-c AMP signal pathway by regulating the content of GS 伪 -PKA-c AMP in the hippocampus and prefrontal cortex of CUMS rats.
【作者单位】：山东中医药大学附属医院;
【基金】：国家自然科学基金资助项目(No.81302956)
【分类号】：R285.5;R-332

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