脑出血免疫抑制大鼠模型的构建及评估

发布时间：2018-05-22 07:15

本文选题：模型 + 动物　；参考：《中国组织工程研究》2016年40期

【摘要】：背景:研究表明,大鼠脑出血后经治疗可以有效抑制其免疫功能。已有文献研究对缺血性脑卒中后免疫抑制现象进行详细研究,但脑出血后机体免疫功能研究鲜见报道。目的:构建脑出血后免疫抑制大鼠模型并进行稳定性评估。方法:将60只Wistar大鼠随机分为3组,即对照组、假手术组、脑出血组,每组20只。采用自身动脉血50μL注入大鼠基底节方法建造急性脑出血模型;假手术模型注入50μL生理盐水,手术过程同脑出血模型;对照组不做任何处理。分别于造模后24,48,72,96 h用血液分析仪检测白细胞、淋巴细胞、淋巴细胞百分数;采用ELISA测定大鼠血清中促炎细胞因子白细胞介素6和抗炎细胞因子转化生长因子β的水平;剖取大鼠脾组织进行组织学及病理学检查;通过RT-PCR及Western blotting检测转化生长因子β、白细胞介素6基因和蛋白在脾组织中的表达变化。结果与结论:(1)与假手术组及对照组相比,脑出血组大鼠于造模后24,48,72,96 h白细胞数明显升高,淋巴细胞百分数逐渐降低,差异有显著性意义(P0.05);(2)与假手术组及对照组相比,脑出血组大鼠血液、脾的白细胞介素6在24 h时高表达,72 h达高峰,96 h时表达降低,差异有显著性意义(P0.05);(3)与假手术组及对照组相比,脑出血组大鼠血液、脾的转化生长因子β于24 h时低表达,72 h逐渐升高,96 h时高表达,差异有显著性意义(P0.05);(4)结果提示,大鼠在脑出血后先呈现免疫功能亢奋,随即96 h后出现免疫功能抑制,说明脑出血后免疫抑制大鼠模型造模成功,并具有良好的稳定性。
[Abstract]:Background: studies have shown that intracerebral hemorrhage after treatment can effectively inhibit its immune function. The phenomenon of immunosuppression after ischemic stroke has been studied in detail, but the study of immune function after cerebral hemorrhage is seldom reported. Objective: to establish an immunosuppressive rat model after intracerebral hemorrhage and evaluate its stability. Methods: 60 Wistar rats were randomly divided into 3 groups: control group, sham operation group and intracerebral hemorrhage group. The acute intracerebral hemorrhage model was established by injecting 50 渭 L autogenous arterial blood into the basal ganglia of rats, and the sham operation model was injected with 50 渭 L normal saline. The percentage of leukocytes and lymphocytes were detected by blood analyzer at 24: 48 and 72 ~ 96 hours after modeling, and the levels of pro-inflammatory cytokine interleukin-6 and anti-inflammatory cytokine transforming growth factor 尾 in serum of rats were measured by ELISA. The changes of expression of transforming growth factor 尾, interleukin 6 gene and protein in spleen tissue were detected by RT-PCR and Western blotting. Results compared with the sham operation group and the control group, the leukocyte count and the lymphocyte percentage in the cerebral hemorrhage group were significantly increased and the lymphocyte percentage was gradually decreased in the cerebral hemorrhage group compared with the sham operation group and the control group (P < 0.05), compared with the sham operation group and the control group. Compared with the sham operation group and the control group, the high expression of interleukin 6 in the spleen decreased at 72 h and reached the peak at 96 h after ICH, and the difference was significant (P 0.05) compared with the sham operation group and the control group, the blood of the ICH group was significantly lower than that of the sham operation group and the control group. The low expression of TGF- 尾 in spleen increased gradually at 72 h after 24 h and increased gradually at 96 h. The difference was significant (P 0.05). The results showed that the immune function of rats was hyperactive after cerebral hemorrhage, and then immunosuppression appeared 96 h later. The results showed that the model of immunosuppressive rats after intracerebral hemorrhage was successful and had good stability.
【作者单位】：天津市第四中心医院神经外科;
【基金】：天津市第四中心医院2014年硕博基金~~
【分类号】：R743.34;R-332

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