激素联合脂多糖诱导股骨头缺血坏死模型兔的骨质变化

发布时间：2018-05-24 20:26

本文选题：股骨头坏死 + 脂多糖类　；参考：《中国组织工程研究》2017年28期

【摘要】：背景:前期研究采用激素联合脂多糖已成功构建股骨头缺血坏死兔模型。目的:进一步观察激素性股骨头坏死早期骨质的动态变化。方法:成年新西兰大白兔24只,随机分为实验组(n=12)和对照组(n=12)。实验组采用激素联合脂多糖改良技术建立激素性股骨头缺血坏死兔模型,对照组相同部位注射等体积生理盐水,饲养条件不变。建模完成后,分别于第5,10,15,20天进行股骨头多层螺旋CT、显微CT及苏木精-伊红染色检测,观察股骨头结构,骨小梁形态、分布,测定骨质密度及空骨陷窝率。结果与结论:(1)影像学结果显示,实验组与对照组兔股骨头形态完整,关节面光滑,第15,20天实验组股骨头骨皮质变薄,骨小梁分布稀疏,间距增宽,不连续,骨矿物质密度、组织矿物质密度、骨体积分数值均显著低于对照组(P0.01);(2)病理学结果显示,实验组空骨陷窝和脂肪细胞不断增多,骨细胞和造血细胞逐渐减少,第15,20天空骨陷窝百分率显著高于对照组(P0.01);(3)结果说明,激素性股骨头坏死早期股骨头内骨细胞坏死增多,伴骨小梁微细骨折发生,导致骨质密度减低,最终可造成骨重塑障碍。
[Abstract]:Background: a rabbit model of avascular necrosis of femoral head has been successfully constructed by hormone and lipopolysaccharide. Objective: to observe the dynamic changes of bone in early stage of steroid-induced femoral head necrosis. Methods: 24 adult New Zealand white rabbits were randomly divided into experimental group (n = 12) and control group (n = 12). The model of steroid-induced avascular necrosis of femoral head was established by hormone and lipopolysaccharide in the experimental group, and the same volume of saline was injected into the control group. After modeling, the multislice spiral CTs of femoral head, microscopic CT and hematoxylin eosin staining were performed on day 5, 10, 15 and 20, respectively. The structure of femoral head, the shape and distribution of trabecular bone, bone density and the rate of empty bone lacuna were observed. Results and conclusion the imaging results showed that the femoral head of the experimental group and the control group had a complete shape and smooth articular surface. On day 1520, the femoral head of the experimental group became thin, the bone trabecular distribution was sparse, the spacing widened, the bone mineral density was discontinuous. The tissue mineral density and bone volume fraction were significantly lower than those in the control group (P 0.01). The pathological results showed that the number of empty bone lacunae and adipocytes in the experimental group was increasing and the number of bone cells and hematopoietic cells was decreasing. The results showed that osteonecrosis of femoral head increased in the early stage of steroid-induced osteonecrosis, accompanied by bone trabecular microfracture, which resulted in the decrease of bone density, which resulted in bone remodeling disorder.
【作者单位】：德阳市人民医院放射科;成都医学院第一附属医院放射科;
【基金】：四川省教育厅资助项目(15ZB0243)~~
【分类号】：R-332;R681.8

【相似文献】