基于~1H-NMR及LC-MS技术研究金铃子散对炎症大鼠模型调节机制的影响

发布时间：2018-05-25 00:44

本文选题：金铃子散 + 抗炎　；参考：《中国中药杂志》2017年02期

【摘要】：为探索金铃子散在治疗炎症过程中对生物体内炎症机制的调节作用,该研究采用了核磁和液质联用技术,将各组大鼠血清样本采用~1H-NMR和LC-MS联用的代谢组学分析方法 ,通过分析角叉菜胶致大鼠足肿胀模型中不同组别炎症大鼠体内代谢物差异的变化,发现并筛选与炎症模型相关的生物标记物,从而推测金铃子散对角叉菜胶致炎的调节机制。通过分析相关数据结果,筛选出与炎症相关的差异性代谢物有18个:鸟苷、9-顺-视黄酸、三磷酸、肌苷5'-磷酸、二磷酸肌苷、三聚磷酸盐、无机磷酸、甘油磷酸胆碱、21-脱氧皮质醇、柠檬酸、谷氨酸、琥珀酸、赖氨酸、牛磺酸、丙酮酸、苹果酸、2-酮戊二酸、甘氨酸等。相关的代谢通路有柠檬酸循环(TCA循环),乙醛酸和二羧酸代谢,甘氨酸,丝氨酸和苏氨酸代谢,丙酮酸代谢。从代谢网络图显示金铃子散在调节机体内炎症方面,可通过调节柠檬酸,琥珀酸,甘氨酸的含量,进而减少氧自由基攻击程度,降低细胞的损伤,从而下调机体组织产生炎性因子起到抗炎的作用。
[Abstract]:In order to explore the effect of Jinlingzi Powder on the mechanism of inflammation in vivo during the treatment of inflammation, this study adopted the technique of nuclear magnetic resonance (NMR) and liquid / mass spectrometry (HPLM). The serum samples of each group of rats were analyzed by the method of combined use of 1H-NMR and LC-MS. By analyzing the changes of metabolites in different groups of inflammatory rats induced by carrageenin in rat foot swelling model, the biomarkers related to the inflammatory model were found and screened, so as to speculate the regulatory mechanism of Jinling Zizian Diangcarrageene-induced inflammation. There were 18 different metabolites related to inflammation: guanosine 9-cis-retinoic acid, triphosphate, inosine 5-phosphoric acid, inosine diphosphate, tripolyphosphate, inorganic phosphoric acid. Glycerol choline phosphate 21-deoxycortisol, citric acid, glutamic acid, succinic acid, lysine, taurine, pyruvate, malic acid 2-ketoglutaric acid, glycine, etc. Related metabolic pathways include citric acid cycle TCA metabolism glyoxylic acid and dicarboxylic acid glycine serine and threonine metabolism pyruvate metabolism. The metabolic network showed that Jinling Zizan could regulate the inflammation of the body by regulating the contents of citric acid, succinic acid and glycine, thereby reducing the degree of oxygen free radical attack and reducing cell damage. Thus down-regulating the body tissue to produce inflammatory factors play an anti-inflammatory role.
【作者单位】：军事医学科学院放射与辐射医学研究所;安徽医科大学;
【基金】：国家自然科学基金项目(21375147);国家自然科学基金青年科学基金项目(81501229) 北京自然科学基金项目(7142125) 国家“重大新药创制”科技重大专项(2012ZX09301003-001-010)
【分类号】：R285.5;R-332

【参考文献】