体外诱导扩增的CD1d限制性人外周血NKT细胞克隆过早凋亡的机理研究

发布时间：2018-05-25 02:25

本文选题：NKT细胞 + 凋亡　；参考：《中国输血杂志》2016年01期

【摘要】：目的针对NKT细胞培养扩增和培养过程中遇到的过早凋亡现象,在凋亡信号层面进行初步的描述和探讨。为今后的NKT相关研发提供数据参考。方法自健康人浓缩白细胞制品(白膜)分离有外周血单个核细胞(peripheral blood mononuclear cells PBMNCs),利用其中的抗原递呈细胞,以α-Gal Cer的刺激NKT细胞克隆产生,记录3-4周NKT的表型变化,和Caspase-3酶活化及细胞凋亡状况。结果刺激7 d后,NKT克隆开始产生,持续扩增3周左右后开始呈现凋亡状态,Caspase3酶在第2周即开始维持高活性状态。人NKT细胞对α-Gal Cer的刺激的反应具有很大的个体差异。结论 NKT细胞克隆可以再最初的3周内如同常规T细胞克隆一样生长,其后则会因α-GalCer刺激引发活化后凋亡。
[Abstract]:Objective to investigate the phenomenon of premature apoptosis in the process of NKT cell proliferation and culture. For the future NKT related research and development to provide data reference. Methods Peripheral blood mononuclear cells (PBMNCs) were isolated from the white membrane of healthy people. The antigen-presenting cells were used to produce NKT cells stimulated by 伪 -Gal Cer, and the phenotypic changes of NKT were recorded at 3-4 weeks. And Caspase-3 enzyme activation and apoptosis. Results after stimulation for 7 days, NKT clones began to produce, and then continued to amplify for about 3 weeks. The apoptotic state of Caspase3 enzyme began to maintain high activity at the second week. The response of human NKT cells to 伪 -Gal Cer is very different. Conclusion NKT cell clone can grow as conventional T cell clone in the first 3 weeks, and then apoptosis after activation induced by 伪 -GalCer stimulation.
【作者单位】：上海市血液中心;
【基金】：上海市自然科学基金面上项目(132R1438900) 上海市卫生局青年科研项目资助,No.024Y37 上海市血液中心科技发展基金项目资助,No.L(9)
【分类号】：R392.12

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