细粒棘球绦虫重组抗原铁蛋白诱导小鼠骨髓树突状细胞免疫应答的机制研究

发布时间：2018-05-25 15:33

本文选题：重组抗原铁蛋白 + 树突状细胞　；参考：《四川大学学报(医学版)》2017年06期

【摘要】：目的探索细粒棘球绦虫重组抗原铁蛋白(rEg.ferritin)免疫小鼠产生抵抗原头蚴感染的保护机制。方法利用rEg.ferritin和囊液(HF)两种细粒棘球绦虫抗原与小鼠骨髓树突状细胞(BMDC)进行体外培养,共分6组:HF组(加入HF 30μg/mL)、rEg.ferritin组(加入抗原rEg.ferritin 1μg/mL)均为单独刺激组,rEg.ferritin+LPS组(加入抗原rEg.ferritin 1μg/mL和LPS 100ng/mL)和HF+LPS组(加入HF 30μg/mL和LPS 100ng/mL)为联合刺激组,LPS阳性对照组(加入LPS 100ng/mL),空白对照组(不加任何抗原)。通过电镜检测各组BMDC形态学的变化;流式细胞技术检测各组DC的表面分子表达、吞噬能力及诱导T细胞增殖能力的变化;酶联免疫吸附试验(ELISA)检测各组BMDC培养上清液中所含Th1型[白介素(IL)-12p70,肿瘤坏死因子-α(TNF-α)]和Th2型(IL-6和IL-10)细胞因子含量。结果 rEg.ferritin组和rEg.ferritin+LPS组BMDC具有成熟的形态,表面突起数目高于空白对照组、HF组和HF+LPS组(P0.05);高表达MHCⅡ、CD80、CD86及CD40分子,与空白对照组比较,差异有统计学意义(P0.05);诱导T细胞增殖能力强于空白对照组、HF组和HF+LPS组(P0.05),吞噬能力弱于HF组;高表达IL-6、IL-12p70、TNF-α及IL-10,与空白对照组比较,差异有统计学意义(P0.05)。结论 rEg.ferritin可以刺激BMDC细胞成熟,促进T增殖,释放高含量的Th1型及Th2型细胞因子,激活Th1型或Th2型细胞,引发免疫应答。
[Abstract]:Objective to explore the protective mechanism of Echinococcus granulosus recombinant antigen ferritin (rEg.ferritin) immunized mice against the infection of the Echinococcus granulosus. Methods using rEg.ferritin and HF two species of Echinococcus granulosus antigen and mouse bone marrow dendritic cells (BMDC) in vitro, they were divided into 6 groups: HF group (adding HF 30 mu g/mL), rEg.ferritin group (plus rEg.ferritin) Antigen rEg.ferritin 1 mu g/mL) was a single stimulation group, group rEg.ferritin+LPS (adding antigen rEg.ferritin 1 g/mL and LPS 100ng/mL) and HF+LPS group (HF 30 mu g/mL and LPS 100ng/mL) were combined stimulation group. Changes in the surface molecular expression of DC, phagocytosis and the ability to induce the proliferation of T cells by flow cytometry; enzyme linked immunosorbent assay (ELISA) to detect the Th1 type of Th1 [IL) -12p70, tumor necrosis factor - alpha (TNF- a)] and Th2 type (IL-6 and IL-10) cytokines in the supernatant of BMDC culture. The number of BMDC in group itin and group rEg.ferritin+LPS was mature, and the number of surface protuberances was higher than that of blank control group, HF group and HF+LPS group (P0.05). The high expression of MHC II, CD80, CD86 and CD40 molecules, compared with the blank control group, was statistically significant (P0.05), and the ability of inducing T cells to proliferate was stronger than that in the blank control group. The strength of IL-6, IL-12p70, TNF- A and IL-10 was higher than that of the HF group. The difference was statistically significant (P0.05) compared with the blank control group (P0.05). Conclusion rEg.ferritin can stimulate the maturation of BMDC cells, promote the proliferation of T, release the high content of Th1 and Th2 cytokine, activate the Th1 type or the type of cell and trigger the immune response.
【作者单位】：宁夏医科大学基础学院医学遗传学与细胞生物学教研室;宁夏医科大学包虫病重点实验室;宁夏医科大学科学技术研究中心;
【基金】：宁夏自然科学基金(No.NZ16208)资助
【分类号】：R392

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