锌指蛋白185在小鼠睾丸支持细胞中的表达与定位

发布时间：2018-05-25 14:31

本文选题：锌指蛋白(ZNF) + 支持细胞　；参考：《现代生物医学进展》2016年18期

【摘要】：目的:检测锌指蛋白185在小鼠睾丸支持细胞中的表达,探讨其与精子发生的关系。方法:提取睾丸和支持细胞总RNA,经半定量RT-PCR法检测ZNF185的转录水平;提取睾丸和支持细胞的蛋白质,利用Western blot分析ZNF185的表达量;制备支持细胞爬片,采用免疫荧光技术检测ZNF185的定位。结果:(1)半定量RT-PCR结果显示:在睾丸支持细胞中扩增出ZNF185基因条带。(2)Western blot结果显示:ZNF185在支持细胞中的表达量显著低于在睾丸组织中的表达量。(3)免疫荧光结果显示:ZNF185主要定位于支持细胞胞质中。结论:ZNF185可能参与支持细胞结构与功能的调控,进而影响精子发生过程。
[Abstract]:Aim: to detect the expression of zinc finger protein 185 in mouse testicular Sertoli cells and to explore the relationship between zinc finger protein 185 and spermatogenesis. Methods: the total RNAs of testis and Sertoli cells were extracted, the transcription level of ZNF185 was detected by semi-quantitative RT-PCR, the protein of testis and Sertoli cells was extracted, and the expression of ZNF185 was analyzed by Western blot. The localization of ZNF185 was detected by immunofluorescence technique. Results the results of semi-quantitative RT-PCR showed that the expression of ZNF185 gene in Sertoli cells was significantly lower than that in Sertoli cells. The immunofluorescence results showed that the expression of ZNF185 gene in Sertoli cells was significantly lower than that in Sertoli cells. The results of immunofluorescence showed that the expression of ZNF185 gene in Sertoli cells was significantly lower than that in testicular tissues. To be located in the cytoplasm of Sertoli cells. Conclusion: ZNF185 may be involved in the regulation of Sertoli cell structure and function, and then affect spermatogenesis.
【作者单位】：潍坊医学院细胞生物学教研室;
【基金】：山东省科技发展计划项目(2015GSF118178) 山东省自然科学基金项目(ZR2013CM032) 山东省卫计委科技基金项目(201411) 潍坊医学院科技创新基金项目(K1302001)
【分类号】：R321

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