2008～2014年中国8省市流行的人呼吸道合胞病毒A亚型G蛋白编码基因全长序列分析

发布时间：2018-05-25 22:05

本文选题：人呼吸道合胞病毒(HRSV) + G蛋白　；参考：《病毒学报》2017年06期

【摘要】：对2008~2014年收集的中国8省市人呼吸道合胞病毒(Human respiratory syncytial virus,HRSV)A亚型87株代表株进行G蛋白编码基因全长核苷酸序列测定和分析,阐明其核苷酸和氨基酸动态变异特征。根据G蛋白第二高变区的靶基因特征,从2008~2014年中国8省市流行的A亚型人呼吸道合胞病毒中挑选代表株,并采用RT-PCR方法对G蛋白编码基因进行扩增和序列测定,通过Sequencher 5.0、MEGA5.05等生物信息学软件进行序列拼接、比对,分析基因亲缘性和氨基酸变异特点。2008~2014年中国8个代表省市收集的296份HRSVA阳性标本,依据靶基因变异程度及特征共挑选出代表株87株,覆盖2008~2014年流行的各基因型,包括GA5,NA4,NA1,NA3,ON1;对87株代表株进行G蛋白编码基因全长序列测定和同源性分析,核苷酸同源性为88.21%~100%,氨基酸同源性为82.09%~100%,与原型株long株的核苷酸和氨基酸同源性分别为89.22%~91.07%和82.77%~86.82%;变异主要集中在第二高变区,其次在胞浆区和第一高变区。根据G蛋白全长核苷酸序列构建亲缘关系进化树,87株代表株可以分为5个分支,与国际上通用的依据G蛋白第二高变区为靶基因进行的基因分型及进化树分支结果一致。本研究系统分析了2008~2014年中国8省市人呼吸道合胞病毒A亚型G蛋白编码基因的基因特征及氨基酸变异情况,为中国人呼吸道合胞病毒的病原学研究,预防和控制提供了重要的病毒学基础研究数据。
[Abstract]:The full-length nucleotide sequence of G protein encoding gene of 87 representative strains of human respiratory syncytial virus (HRRSV) from 2008 to 2014 in 8 provinces and cities of China were determined and analyzed, and the dynamic variation characteristics of nucleotide and amino acid were elucidated. According to the target gene characteristics of the second high variable region of G protein, the representative strains of human respiratory syncytial virus A subtype A were selected from 8 provinces and cities of China from 2008 to 2014. The G protein encoding gene was amplified and sequenced by RT-PCR. Sequencher 5.0 MEGA 5.05 and other bioinformatics software were used to sequence splice and compare the genetic relationship and amino acid variation characteristics. From 2008 to 2014, 296 HRSVA positive specimens were collected from 8 representative provinces and cities in China. A total of 87 representative strains were selected according to the degree and characteristics of target gene variation, covering all the prevalent genotypes from 2008 to 2014, including GA5, NA4, NA1, NA1, NA3ON1.The full-length sequence and homology analysis of G-protein coding genes were performed on 87 representative strains. The homology of nucleotide and amino acid was 88.21 and 82.09100, respectively, and the homology of nucleotide and amino acid with long strain was 89.222.22% and 82.77%, respectively. The variation was mainly concentrated in the second high variable region, followed by the cytoplasmic region and the first high variable region. According to the full-length nucleotide sequence of G protein, 87 representative strains of phylogenetic tree can be divided into 5 branches, which are consistent with the results of the universal genotyping and phylogenetic tree branches based on the target gene of the second high variable region of G protein. This study systematically analyzed the gene characteristics and amino acid variation of subtype A G protein encoding gene of human respiratory syncytial virus A in 8 provinces and cities of China from 2008 to 2014, which is the etiological study of Chinese respiratory syncytial virus. Prevention and control provide important basic virology research data.
【作者单位】：中国疾病预防控制中心病毒病预防控制所卫生部医学病毒学和病毒病重点实验室世界卫生组织西太平洋区麻疹/风疹参比实验室;北京市大兴区鹿圈社区卫生服务中心;广州医科大学第一附属医院呼吸疾病国家重点实验室广州呼吸疾病研究所;长春市儿童医院;甘肃省疾病预防控制中心;陕西省疾病预防控制中心;湖南省疾病预防控制中心;安徽理工大学医学院;
【基金】：国家自然科学基金(项目号:81772195),题目:我国大陆流行的人呼吸道合胞病毒F蛋白抗原位点动态变异研究国家重大科技专项课题(项目号:2013ZX10004202),题目:艾滋病和病毒性肝炎等重大传染病防治~~
【分类号】：R373

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