不同保存液对供肺抗炎抗氧化能力及病理超微结构的影响

发布时间：2018-05-26 22:14

本文选题：FCE + 肺保护　；参考：《暨南大学》2012年硕士论文

【摘要】：目的：研究乳化氟碳(Perfluorocarbon Emulsions, FCE)、UW液(the University ofWisconsin solution, UWs)及二者混合液对大鼠离体肺不同保存期抗炎抗氧化能力及病理超微结构的影响，以肺组织氧化损伤程度、炎症因子表达水平及病理、超微结构改变作为观察指标，综合比较三种液体对离体肺脏保存期肺损伤的保护效果，从而为临床寻找一种安全有效的供肺保存方法。方法：将72只SD大鼠随机分为6组，每组12只，所有大鼠建立离体肺灌注模型后取下心肺，保存于肺保存液中。UW-6及UW-12组采用UW液灌注与保存，分别保存6小时与12小时，UW/FCE-6及UW/FCE-12组采用UW液与FCE的混合液灌注与保存，保存时间同上，FCE-6、FCE-12组采用FCE灌注与保存，保存时间同上。取肺组织检测氧化程度、炎症因子水平、组织病理学改变并评分、超微结构变化。结果：（1）三组保存液组间IL-1β、IL-6及TNF-α水平的比较均无统计学意义（P0.05）；（2）三组保存液6小时IL-1β、IL-6及TNF-α水平与12小时相比均无统计学意义（P0.05）；（3）三组保存液6小时MDA、MPO含量及病理积分显著低于12小时（P0.05），，SOD活性则显著高于12小时（P0.05）；（4）FCE组SOD活性显著高于UW/FCE混合液组（P0.05），UW/FCE混合液组MDA含量显著高于余两组（P0.05），FCE组MPO含量及病理积分显著低于余两组（P0.05）；（5）FCE组在病理改变及超微结构改变上均较轻微，而UW及UW/FCE组则较重。结论：与UW液相比，乳化氟碳也具显著抗氧化能力，但二者混合液抗氧化能力最差；乳化氟碳在抑制炎症细胞聚集方面具有明显优势，能更好的延缓及减轻缺血保存期的肺损伤。故我们认为，乳化氟碳也可单独作为供肺保存液。
[Abstract]:Objective: To study the effects of emulsified perfluorocarbon Emulsion (University ofWisconsin solution, UWs) and its mixture on anti-inflammatory and antioxidation ability and pathological ultrastructure of rat lungs during different preservation periods, the degree of oxidative injury, the expression level of inflammatory factors and the pathology of lung tissue were studied. Ultrastructural changes were used as an observation index to compare the protective effects of three liquids on lung injury in vitro, so as to find a safe and effective method for clinical preservation of donor lung. Methods: 72 Sprague-Dawley rats were randomly divided into 6 groups, 12 rats in each group. All the rats were taken out of heart and lung after the model of isolated lung perfusion was established, and stored in lung preservation solution. UW-6 and UW-12 groups were perfused and preserved with UW solution. The UW / FCE-6 group and the UW/FCE-12 group were perfused with the mixture of UW solution and FCE for 6 hours and 12 hours, respectively. The storage time was the same as that in the FCE-6FCE-12 group, and the preservation time was the same as that in the same time. The degree of oxidation, the level of inflammatory factors, the histopathological changes and the ultrastructural changes were measured. Results: (1) there was no significant difference in the levels of IL-1 尾 -IL-6 and TNF- 伪 between the three groups. There was no significant difference in the levels of IL-1 尾 -IL-6 and TNF- 伪 between the three groups. At 12 hours, the activity of SOD in the P0.05FCE group was significantly higher than that in the UW/FCE mixture group (P0.05 / FCE-FCE group), while the content of MPO and the pathological score in the P0.05FCE group were significantly higher than those in the other two groups (P0.05P0.05FCE group) and the pathological changes and the ultrahigh level of MDA content in the other two groups were significantly lower than those in the other two groups. The microstructural changes were slight. The UW and UW/FCE groups were more severe. Conclusion: Compared with UW solution, emulsified fluorocarbon also has obvious anti-oxidation ability, but the anti-oxidation ability of the mixed solution is the worst. Emulsified fluorocarbon has obvious advantages in inhibiting inflammatory cell aggregation, and can better delay and reduce lung injury during ischemic preservation. Therefore, we believe that emulsified fluorocarbon can also be used as donor lung preservation solution alone.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

【相似文献】