应用骨髓基质细胞片层构建组织工程骨的实验研究

发布时间：2018-05-26 21:34

  本文选题：骨髓基质细胞 + 细胞片层　； 参考：《青岛大学》2011年硕士论文

【摘要】：目的探讨犬骨髓基质细胞(bone marrow stromal cells, BMSCs)细胞片层在构建组织工程骨中的作用。 方法制备犬同种异体脱钙骨基质(dermineralized bone matrix, DBM)。将人重组骨形态发生蛋白-2(recombination human bone morphogenetic protein-2, rhBMP-2)复合到DBM上。抽取犬髂骨骨髓,采用密度梯度离心法分离犬骨髓基质细胞(BMSCs),将其向成骨细胞诱导分化。将经成骨诱导的第3代细胞接种于温度反应性培养皿中培养,至细胞增殖并互相融合时,将培养箱温度降至20℃,制备完整的BMSCs细胞片层,将其包裹DBM/rhBMP-2/BMSCs复合体植入犬左侧背阔肌下包裹胸背动静脉束为实验侧,以无BMSCs细胞片层包裹的DBM/rhBMP-2/BMSCs复合体植入犬右侧背阔肌下包裹胸背动静脉束为对照侧。术后8、12、16周取材行大体观察、影像学观察及组织学观察,评价成骨情况。采用SPSS18.0统计软件对数据进行两样本均数差别的t检验。 结果 1.制备的BMSCs细胞片层完整致密,在支架复合体表面生长良好。 2.成骨面积实验侧对照侧,两者之间差异有显著性(P0.05)。术后16周,实验侧有大量板层骨形成,有哈弗氏系统形成,骨髓腔内有红骨髓；对照侧有板层骨形成,无哈弗氏系统形成,骨髓腔内无红骨髓。 结论BMSCs细胞片层可促进具有致密板层骨和哈弗氏系统的组织工程骨的形成。
[Abstract]:Objective to investigate the role of bone marrow stromal cells, BMSCs) cell lamellae in the construction of tissue engineered bone in canine bone marrow stromal cells. Methods canine allogeneic demineralized bone matrix (DBM) was prepared. Human recombinant bone morphogenetic protein-2 recombination human bone morphogenetic protein-2 (rhBMP-2) was synthesized into DBM. The bone marrow of iliac bone of dogs was extracted and the bone marrow stromal cells (BMSCs) were isolated by density gradient centrifugation and differentiated into osteoblasts. The third passage cells induced by osteogenesis were cultured in a temperature-reactive culture dish. When the cells proliferated and fused with each other, the incubator temperature was lowered to 20 鈩，

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