NKT细胞发育过程中microRNA表达谱变化

发布时间：2018-05-28 20:00

本文选题：自然杀伤性T细胞 + microRNA　；参考：《中国免疫学杂志》2017年07期

【摘要】：目的:探讨自然杀伤性T细胞(NKT)发育、成熟过程中microRNA表达谱变化。方法:采用流式细胞仪分选小鼠胸腺不同发育阶段NKT细胞,提取细胞总RNA,经反转录和预扩增后利用Taq Man低密度microRNA表达谱分析阵列检测NKT发育、成熟过程中发生表达变化的microRNAs,并采用real-time PCR进一步验证。结果:NKT细胞发育、成熟过程中,共有92个microRNAs表达发生显著变化。表达显著增加的microRNAs有71个,其中有36个表达持续增加;而表达显著降低的microRNAs有21个,其中有12个表达持续降低。选取Let-7f、miR-150、miR-155、miR-223、miR-24和miR-29进行real-time PCR,发现Let-7f、miR-150、miR-24、miR-29在NKT细胞发育、成熟过程中表达增加,而miR-223和miR-155表达降低,其表达变化趋势与表达谱分析一致。结论:NKT细胞发育、成熟过程中伴随大量特异microRNAs的不同表达变化,提示特异microRNA调控NKT细胞的发育和功能。
[Abstract]:Objective: to investigate the changes of microRNA expression profile during the development and maturation of natural killer T cells. Methods: NKT cells were isolated from mouse thymus at different stages of development by flow cytometry. After reverse transcription and preamplification, the development of NKT was detected by Taq Man low density microRNA expression analysis array. The expression of microRNAsduring maturation was further verified by real-time PCR. Results 92 microRNAs expressions were significantly changed during the development and maturation of the cell line. There were 71 microRNAs with significant increase in expression, 36 of which continued to increase, while 21 were significantly decreased in microRNAs, 12 of which continued to decrease. The Let-7ftmiR-150miR-150miR-155 miR-223miR-24 and miR-29 were selected to carry out real-time PCR. It was found that the expression of Let-7fmiR-150 miR-150 miR-24 miR-29 increased during the development of NKT cells, while the expression of miR-223 and miR-155 decreased. The trend of expression change was consistent with the analysis of expression profile. Conclusion the different expression of specific microRNAs in the mature process of microRNAs suggests that specific microRNA regulates the development and function of NKT cells.
【作者单位】：华北理工大学基础医学院河北省慢性疾病重点实验室唐山市慢性病临床基础研究重点实验室;北京大学医学部实验动物科学部;华北理工大学附属医院血液病学科;
【基金】：国家自然科学基金面上项目(81373111)资助
【分类号】：R392

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