氯胺酮对发育期大鼠的认知功能及大脑海马区nNOS表达的影响

发布时间：2018-05-28 19:13

本文选题：氯胺酮 + 认知功能　；参考：《吉林大学》2011年硕士论文

【摘要】：本研究采用Y-迷宫测试及免疫组织化学染色法观察氯胺酮对发育期大鼠学习记忆功能及大脑海马区nNOS表达的影响,初步探讨氯胺酮对大鼠学习记忆功能的影响及其作用机制。本实验通过对随机分组的SD大鼠腹腔注射不同剂量的氯胺酮建立全身麻醉动物实验模型,采用Y-迷宫行为学测试法观察和记录达标所需训练次数、正确反应率及主动回避率作为学习记忆功能的评估指标;行为学测试完成后采用免疫组织化学染色法检测各组大鼠海马区nNOS的表达情况。氯胺酮麻醉期间各组SD大鼠的脉搏、氧饱和度等生命体征基本稳定；Y-迷宫测试学习记忆功能结果显示,达标所需训练次数、正确反应率和主动回避率在低剂量组和训练组之间比较差异无显著性；高剂量组达标所需训练次数明显高于其他组(除第4天外)；高剂量组正确反应率明显低于其他组；高剂量组主动回避率明显低于训练组,但与低剂量组相比差异无显著性。免疫组织化学染色结果显示空白组nNOS基本无表达,高剂量组nNOS表达明显低于训练组和低剂量组,低剂量组与训练组相比无明显差异。实验证明,多次高剂量氯胺酮注射麻醉对发育期大鼠的学习记忆功能具有损害作用,这种损害作用可能与大脑海马区nNOS表达的抑制有关。
[Abstract]:In this study, the effects of ketamine on learning and memory function and nNOS expression in hippocampus of developing rats were observed by means of Ymaze test and immunohistochemical staining, and the effects of ketamine on learning and memory function and its mechanism were studied. In this experiment, a general anesthetic animal model was established by intraperitoneal injection of ketamine at different doses to randomly divided SD rats, and the training times required to meet the standard were observed and recorded by using Y- maze behavioral test method. The correct response rate and active avoidance rate were used to evaluate the learning and memory function, and the expression of nNOS in hippocampus of rats in each group was detected by immunohistochemical staining after behavioral test. During ketamine anesthesia, the vital signs such as pulse and oxygen saturation of SD rats in each group were basically stable. The correct response rate and active avoidance rate were not significantly different between the low dose group and the training group, the training times of the high dose group were significantly higher than those of the other groups (except the 4th day), the correct response rate of the high dose group was significantly lower than that of the other groups. The active avoidance rate in the high dose group was significantly lower than that in the training group, but there was no significant difference between the high dose group and the low dose group. The results of immunohistochemical staining showed that there was no expression of nNOS in the blank group, but the expression of nNOS in the high dose group was significantly lower than that in the training group and the low dose group, but there was no significant difference between the low dose group and the training group. It is proved that multiple high dose ketamine injection anesthesia can damage the learning and memory function of the developing rats, which may be related to the inhibition of nNOS expression in the hippocampus.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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