B型流感病毒小鼠致死模型的建立

发布时间：2018-05-30 04:22

本文选题：流感病毒 + B型　；参考：《军事医学》2017年01期

【摘要】：目的建立B型流感病毒致死小鼠动物模型,为研究B型流感病毒的致病与传播机制、开发新疫苗和抗病毒药物奠定基础。方法将病毒B/Lee/1940在小鼠体内连续传代5次后,获得小鼠致死性病毒适应株。利用MDCK细胞扩增的小鼠致死性病毒适应株感染小鼠,连续监测14 d小鼠体质量变化和死亡情况。同时对P0代病毒和P5代小鼠致死性病毒适应株的8个基因片段(PB2,PB1,PA,HA,NA/NB,NP,M,NS)进行序列分析。结果与结论传代过程中每一代小鼠肺中都可检测到病毒;感染致死性病毒适应株的小鼠体质量变化剧烈,小鼠死亡率为100%,并且在小鼠肺中能检测到病毒。测序分析发现小鼠致死性病毒适应株在PB2、NP片段的部分位点发生了氨基酸突变。该研究通过将病毒在小鼠体内连续传代成功建立了B型流感病毒小鼠致死模型,为B型流感病毒的研究奠定了基础。
[Abstract]:Objective to establish a murine model of influenza B virus death in order to study the pathogenicity and transmission mechanism of influenza B virus and to develop new vaccines and antiviral drugs. Methods the virus B/Lee/1940 was subcultured in mice for 5 times, and the lethal virus adaptation strain was obtained. The mice infected with the lethal virus acclimated by MDCK cells were monitored for 14 days of body mass change and death. At the same time, eight gene fragments of P0 generation virus and P5 generation mouse lethal virus adaptation strain were sequenced. Results and conclusion virus could be detected in the lungs of each generation of mice during passage, and the body mass of mice infected with the lethal virus adaptation strain changed dramatically, the mortality rate of mice was 100%, and the virus could be detected in the lungs of mice. Sequence analysis showed that amino acid mutation occurred in some sites of NP fragment of PB2. In this study, the murine lethal model of influenza B virus was successfully established by successive subculture of the virus in mice, which laid a foundation for the study of influenza B virus.
【作者单位】：天津科技大学;中国科学院天津工业生物技术研究所;中国科学院流感研究与预警中心;
【基金】：中国科学院科技攻关项目(KJZD-ZW-L09)
【分类号】：R511.7;R-332

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