仙茅苷对血管性痴呆模型大鼠海马区Caspase-3、PARP-1和雌激素受体表达的作用

发布时间：2018-05-30 06:44

本文选题：仙茅苷 + 血管性痴呆　；参考：《神经解剖学杂志》2017年04期

【摘要】：目的:研究仙茅苷对血管性痴呆(VD)模型大鼠海马区Caspase-3、PARP-1和雌激素受体(ER)表达的作用。方法:确立SD大鼠对照组后,用构建的血管性痴呆模型大鼠随机分成模型组、药物仙茅苷低(24 mg/kg)和高剂量(72 mg/kg)组(予以仙茅苷灌胃4周),每组8只。用药结束后,按设计用Morris水迷宫测试大鼠空间学习记忆功能;流式细胞术分析海马区神经细胞元凋亡;Caspase-3、PARP-1和ER蛋白水平用Western Blot测定;Caspase-3、PARP-1和ER mRNA表达用Realtime PCR检测。结果:Morris水迷宫试验结果显示,药物组和模型组大鼠逃避潜伏期均明显延长(P0.05~0.01),模型组大鼠更明显(P0.01);药物组和模型组大鼠空间探索距离百分比均降低(P0.05~0.01),模型组大鼠降更明显(P0.01)。与对照组比较,药物组和模型组大鼠海马神经细胞凋亡率显著提高(P0.01),模型组大鼠更明显(P0.01)。与对照组比较,模型组的Caspase-3、PARP-1蛋白和mRNA表达均增加(P0.05);与模型组相比,仙茅苷各剂量组可见ER表达增加(P0.05),Caspase-3和PARP-1表达降低(P0.05)。与对照组比较,模型组的Caspase-3和PARP-1表达增加(P0.01),而ER未见明显变化(P0.05);与模型组相比,仙茅苷各剂量组ER表达均增加(P0.01),Caspase-3和PARP-1表达不明显(P0.05),但不同剂量仙茅苷组间未见明显差异(P0.05)。结论:结果表明仙茅苷具有改善血管性模型大鼠空认知功能的作用是通过抑制海马区神经细胞凋亡,下调Caspase-3和PARP-1表达,上调海马ER表达实现的。
[Abstract]:Aim: to study the effect of citronelin on the expression of Caspase-3, PARP-1 and estrogen receptor (ERR) in hippocampus of vascular dementia rats. Methods: Sprague-Dawley rats (SD rats) were randomly divided into two groups: the model group (24 mg / kg) and the high dose group (72 mg / kg). After treatment, the spatial learning and memory function of rats was measured by Morris water maze, and the expression of Caspase-3, PARP-1 and ER mRNA in hippocampal neurons were analyzed by flow cytometry. The expression of Caspase-3, PARP-1 and ER mRNA was detected by Realtime PCR. Results the results of the water maze test showed that the escape latency of both the drug group and the model group was significantly longer than that of the model group, and that of the model group was more obvious than that of the model group, and that of the drug group and the model group was significantly lower than that of the drug group and the model group, and the percentage of the space exploration distance of the drug group and the model group was lower than that of the model group, and that of the model group was more obvious than that of the model group. Compared with the control group, the apoptotic rate of hippocampal neurons in the drug group and the model group was significantly increased, and that in the model group was more obvious than that in the model group. Compared with the control group, the expression of Caspase-3, PARP-1 and mRNA increased in the model group, while the expression of ER increased and the expression of Caspase-3 and PARP-1 decreased in the model group compared with the model group. Compared with the control group, the expression of Caspase-3 and PARP-1 increased in the model group, but there was no significant change in ER. Compared with the model group, the expression of Caspase-3 and PARP-1 was not significantly increased in each dose group, but there was no significant difference between the different dose groups. Conclusion: the results suggest that citronelin can improve the spatial cognitive function of vascular model rats by inhibiting the apoptosis of hippocampal neurons, down-regulating the expression of Caspase-3 and PARP-1, and up-regulating the expression of ER in hippocampus.
【作者单位】：庆阳市人民医院神经内科;陇东学院岐伯医学院;解放军169医院消化血液科;西安医学院基础医学研究所;
【基金】：国家自然科学基金(81471265) 陕西省科技统筹创新工程计划项目(2012KTCG01-02)
【分类号】：R285.5;R-332

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