不同代次人脐带间充质干细胞的生物学特性及分化

发布时间：2018-05-30 21:36

本文选题：干细胞 + 脐带　；参考：《中国组织工程研究》2017年13期

【摘要】：背景:人脐带间充质干细胞作为组织工程及再生医学领域的新兴种子细胞,其应用价值有赖于能否在长期体外传代扩增后仍保持稳定生物学特性和多向分化能力,需要进一步的实验研究。目的:研究不同培养代次人脐带间充质干细胞重要生物学属性的差异。方法:采用组织块法分离培养人脐带间充质干细胞,分别采用细胞计数法与MTT法检测第3-6代细胞增殖活性;流式细胞仪检测第1-9代细胞表面标志物;细胞染色法分析第3-6代细胞核型。取第3代人脐带间充质干细胞,检测其分化为神经细胞、成骨细胞及肝样细胞的能力。结果与结论:(1)细胞计数法与MTT方法检测结果一致,第3-6代脐带间充质干细胞的增殖曲线均呈"S"型,在一二天时处于缓慢增长期,2-4 d时处于对数增长期,四五天时处于平台期,五六天时处于衰退期;(2)第1-9代脐带间充质干细胞表面高表达CD90、CD44、CD73,不或低表达CD11b、CD18、CD34、CD43及HLA-DR;(3)第3-6代脐带间充质干细胞核型均为正常人体核型;(4)脐带间充质干细胞可分化为神经细胞、成骨细胞及肝样细胞;(5)结果表明,人脐带间充质干细胞具有高度增殖活性和多向分化特性。
[Abstract]:Background: as a new seed cell in tissue engineering and regenerative medicine, the application value of human umbilical cord mesenchymal stem cells depends on the ability to maintain stable biological characteristics and multidirectional differentiation after long-term passage and amplification in vitro. Further experimental research is needed. Objective: to study the difference of important biological properties of human umbilical cord mesenchymal stem cells in different generations. Methods: human umbilical cord mesenchymal stem cells were isolated and cultured by tissue mass method. The proliferative activity of passage 3-6 cells was detected by cell count method and MTT method, and cell surface markers of passage 1-9 were detected by flow cytometry. The nuclear types of passage 3-6 were analyzed by cell staining. The third generation of human umbilical cord mesenchymal stem cells were taken and their ability to differentiate into neural cells, osteoblasts and hepatoid cells was tested. The results were consistent with those of MTT method. The proliferation curve of umbilical cord mesenchymal stem cells of passage 3 to 6 was "S" type, and it was in logarithmic growth phase at 2 ~ 4 days after slow growth on day 1 and 2, and at platform stage on day 45. During the 5th and 6th days, the mesenchymal stem cells of the umbilical cord expressed CD90, CD4, CD73, CD11, CD18, CD34, CD43 and HLA-DR3, respectively. The nuclear types of the mesenchymal stem cells of the 3rd and 6th generations of cord were all normal human karyotype and the mesenchymal stem cells could differentiate into neural cells, and the mesenchymal stem cells of the umbilical cord could differentiate into neural cells, and the mesenchymal stem cells of the third and sixth generations could differentiate into neural cells. The results of osteoblasts and hepatoid cells showed that human umbilical cord mesenchymal stem cells had high proliferative activity and multidirectional differentiation.
【作者单位】：贵州医科大学组织工程与干细胞实验中心;贵州医科大学基础医学院免疫学教研室;贵州医科大学儿童医学中心;贵州医科大学实验动物中心;
【基金】：贵州省“125计划”重大科技专项(黔教合重大专项字[2013]020)资助~~
【分类号】：R329.2

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