面向现场分析的微流控免疫分析系统的研究

发布时间：2018-06-02 02:04

本文选题：微流控学 + 免疫　；参考：《浙江大学》2012年硕士论文

【摘要】：免疫分析(Immunoassay)是利用抗原和抗体间的特异性反应对目标分析物进行检测的一种方法。由于其极高的特异性和灵敏度,在环境监测、食品卫生、生化分析等领域都有广泛的应用。但是,常规的免疫分析孵育时间长,从采集样品到获得结果需一天到数天时间；并且,此法加样繁琐,要求操作规范,只能由专业人员完成,目前主要应用于医院或者医疗站的实验室。微流控学(Microfluidics)是上世纪九十年代发展起来的在微米级结构中操控纳升至微升级流体的科学和技术。由于其特殊的微尺度效应以及显著提高的比表面积,微分析系统具有耗样小、易集成、费时短等优点,适用于现场分析,目前已广泛应用于便携式免疫分析仪的研究。第一章对近年来微流控免疫分析系统进行了综述,尤其对系统中采用的驱动方式以及液体操控技术进行了重点介绍。第二章建立了一种基于试剂预封装技术和程序化微蠕动泵控制技术的面向现场分析的微流控免疫分析系统。该系统采用油相间隔各种分析试剂,预封装于Teflon管内,并采用自制的程序化微蠕动泵作为驱动设备,构建了一种一步法微流控免疫分析系统。实验将毛细管进样端插入样品,按下控制键,系统自动吸入1μL样品,之后样品和所有分析试剂被依次推出并流经毛细管免疫反应柱,完成免疫反应和洗涤步骤,一步实现从进样到分析的全过程。由于采用油相间隔,分析过程中各试剂间未发生混合。单独考察了微蠕动泵驱动下的液流流速的稳定性,70min内流速的RSD为1.2%(n=35)。为了考察该系统的性能,检测了不同浓度的人类IgG,检测限为0.68μg/mL,动态监测范围为0-300μg/mL。连续平行测定5次,毛细管荧光强度的RSD为2.4%(n=5)。此系统尺寸小、造价低廉、性能稳定、无操作人员限制,充分展示了其应用于现场分析的潜力。
[Abstract]:Immunoassay is a method for the detection of target analytes using specific reactions between antigens and antibodies. Because of its high specificity and sensitivity, it has been widely used in environmental monitoring, food hygiene, biochemical analysis and other fields. However, the incubation time of routine immunoassay is long, from collecting samples to getting the results takes one to several days. Moreover, this method is complicated and requires standard operation, which can only be completed by professionals. At present, it is mainly used in the laboratory of hospital or medical station. Microfluidic (Microfluidic) is a science and technology developed in the 1990s to manipulate nano-to-micro-upgrading fluid in a micro-scale structure. Due to its special microscale effect and remarkable increase in specific surface area, the microanalysis system has the advantages of small sample consumption, easy integration and short time consumption. It is suitable for field analysis and has been widely used in the research of portable immune analyzer. In the first chapter, the microfluidic immunoassay system is reviewed in recent years, especially the driving mode and liquid manipulation technology used in the system. In the second chapter, a microfluidic immunoassay system for field analysis is established based on reagent prepackaging and programmed microperistaltic pump control. In this system, a one-step microfluidic immunoassay system was constructed by using oil-phase separation reagent, pre-encapsulated in Teflon tube, and self-made programmable micro-creeping pump as driving device. In the experiment, the capillary sample was inserted into the sample, the control key was pressed, and the system automatically inhaled the 1 渭 L sample. After that, the sample and all the analytical reagents were pushed out in turn and flowing through the capillary immunoreaction column to complete the immune reaction and washing steps. The whole process from sample injection to analysis is realized in one step. Due to the oil phase interval, there was no mixing between the reagents during the analysis. The stability of liquid flow velocity driven by micro-peristaltic pump was investigated separately. The RSD of flow velocity within 70 minutes was 1.2 ~ (35). In order to investigate the performance of the system, the detection limit of human IgG was 0.68 渭 g / mL and the dynamic monitoring range was 0-300 渭 g / mL. The RSD of capillary fluorescence intensity was 2. 4%. The system is small in size, low in cost, stable in performance and no limitation for operators, which fully demonstrates its potential application in field analysis.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R392

【共引文献】