重楼总皂甙对小鼠哮喘模型气道炎症的影响及机制

发布时间：2018-06-02 13:38

本文选题：哮喘 + 重楼总皂甙　；参考：《中国老年学杂志》2017年19期

【摘要】：目的探讨重楼总皂甙对小鼠哮喘模型的保护作用及可能机制。方法将24只BALB/c小鼠随机分为空白对照组,模型组,重楼总皂甙低剂量组(LRTPS)和重楼总皂甙高剂量组(HRTPS),每组6只。除空白对照组小鼠外,其余各组采用卵蛋白致敏建立支气管哮喘小鼠模型,治疗组在造模的同时灌胃给予不同剂量的重楼总皂甙治疗。通过肺组织病理切片、酶联免疫反应(ELISA)法检测肺泡灌洗液中的白细胞介素(IL)-4和干扰素(IFN)-γ水平,Q-PCR检测肺组织IL-4和IFN-γmRNA表达水平。结果空白对照组小鼠肺组织支气管无炎性细胞浸润,气道无明显黏液分泌,模型组支气管和血管周围炎性细胞浸润明显,黏膜及黏膜下层水肿明显。治疗组支气管和血管周围炎性细胞浸润明显减少,肺泡结构清晰,肺泡腔和支气管腔无渗出物。哮喘组小鼠的肺泡灌洗液IL-4水平和肺组织IL-4 mRNA表达显著高于对照组;治疗组显著低于哮喘组(P0.05)。哮喘组小鼠的肺泡灌洗液IFN-γ水平和肺组织IFN-γmRNA表达显著低于对照组,治疗组显著高于哮喘组(P0.05)。结论重楼总皂甙能明显减轻哮喘气道炎性反应,其机制可能与恢复局部Th1/Th2细胞因子平衡有关。
[Abstract]:Objective to investigate the protective effect and possible mechanism of total saponins in mice with asthma. Methods Twenty-four BALB/c mice were randomly divided into control group, model group, low dose group and high dose group with 6 rats in each group. In addition to the blank control group, the other groups were sensitized with ovalbumin to establish the model of bronchial asthma. The treatment group was treated with different doses of total saponins in the stomach at the same time. The levels of IL-4 and IFN- 纬 in alveolar lavage fluid were detected by enzyme-linked immunosorbent assay (Elisa). Q-PCR was used to detect the expression of IL-4 and IFN- 纬 mRNA in lung tissue. Results there were no inflammatory cells infiltration and mucus secretion in the bronchus of the blank control group. In the model group, the infiltration of inflammatory cells in the bronchus and perivascular was obvious, and the edema of mucosa and submucous membrane was obvious in the model group. In the treatment group, the infiltration of inflammatory cells in the bronchi and blood vessels was decreased, the alveolar structure was clear, and there was no exudate in the alveolar and bronchial lumen. The level of IL-4 in alveolar lavage fluid and the expression of IL-4 mRNA in lung tissue in asthmatic group were significantly higher than those in control group, while those in treatment group were significantly lower than those in asthma group. The level of IFN- 纬 in alveolar lavage fluid and the expression of IFN- 纬 mRNA in lung tissue in asthma group were significantly lower than those in control group, and the level of IFN- 纬 in treatment group was significantly higher than that in asthma group. Conclusion the total saponins can significantly reduce the airway inflammatory response of asthma, and the mechanism may be related to the restoration of local Th1/Th2 cytokine balance.
【作者单位】：吉首大学医学院;湘西自治州人民医院;
【基金】：国家自然科学基金(No.81560038,81460766) 湖南省教育厅青年项目(No.13B090) 吉首大学大学生创新性项目(JSUCX-2013-28) 湖南省卫生厅项目(B2014-184)
【分类号】：R285.5;R-332

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