补肾益智方对D-半乳糖联合β-淀粉样蛋白25-35致老年痴呆模型大鼠的作用及机制

发布时间：2018-06-02 12:27

本文选题：动物 + 模型　；参考：《中国组织工程研究》2016年49期

【摘要】：背景:中药复方具有多靶点的作用特点,能通过多个环节对中枢胆碱能神经系统进行调节,有效改善患者的认知功能。研究证明,补肾益智方可以改善老年痴呆患者的症状,提高生活质量。目的:探讨补肾益智方对D-半乳糖联合β-淀粉样蛋白25-35致老年痴呆模型大鼠的作用及其机制。方法:选用健康成年SD大鼠,随机分为对照组、模型组、阳性药物组(多奈哌齐组0.3 g/kg)、补肾益智方20 g/kg组及补肾益智方5 g/kg组,每组10只,采用皮下注射D-半乳糖联合双侧海马注射β-淀粉样蛋白25-35构建痴呆大鼠模型。用补肾益智方药液灌胃治疗8周后,懫用Morris水迷宫测试检测大鼠的学习记忆能力;采用高频刺激Schaffer侧支,在同侧海马CA1区诱导长时程增强的方法检测大鼠海马神经元突触可塑性的变化;同时检测大鼠脑组织多种指标,包括超氧化物歧化酶活性、丙二醛、单胺氧化酶B、胆碱乙酰基转移酶、乙酰胆碱酯酶、肿瘤坏死因子α、白细胞介素1等。结果与结论:(1)水迷宫实验中补肾益智方组可显著改善大鼠的学习记忆能力,模型组与对照组相比海马CA1区长时程增强幅度显著降低(P0.05);(2)补肾益智方5 g/kg组与模型组比较,群体峰电位幅度差异无显著性意义;补肾益智方20 g/kg组群体峰电位幅度明显高于模型组和补肾益智方5 g/kg组,能减轻D-半乳糖联合β-淀粉样蛋白25-35对海马CA1区长时程增强的抑制作用,改善突触功能的可塑性;(3)补肾益智方组能显著降低肿瘤坏死因子α和白细胞介素1的水平(P0.05),明显提高胆碱乙酰基转移酶和超氧化物歧化酶活性(P0.05),降低乙酰胆碱酯酶活性及丙二醛水平(P0.05);(4)结果提示,补肾益智方对阿尔茨海默病大鼠模型学习记忆的改善作用可能与抑制脑内炎症反应、调节胆碱能系统、抗氧化作用等有关。
[Abstract]:Background: Chinese medicine compound has the characteristics of multiple targets, can regulate the central cholinergic system through several links and effectively improve the cognitive function of the patients. The study has proved that the prescription of tonifying kidney and nourishing wisdom can improve the symptoms of Alzheimer's patients and improve the quality of life. Objective: To explore the combination of D- galactose and beta amyloid eggs with the tonifying kidney and Yizi prescription. The effect and mechanism of white 25-35 induced Alzheimer's model rats. Methods: the healthy adult SD rats were randomly divided into control group, model group, positive drug group (0.3 g/kg of donepezil group), group of 20 g/kg of tonifying Yizi Fang and 5 g/kg group of tonifying kidney Yizi prescription, 10 rats in each group, by subcutaneous injection of D- galactose combined with bilateral hippocampal injection of beta amyloid eggs with bilateral hippocampus The model of dementia rat was constructed in white 25-35. After 8 weeks of gavage, the learning and memory ability of rats was detected by Morris water maze test. The changes of synaptic plasticity in hippocampal neurons of rats were detected by high frequency stimulation of the Schaffer lateral branch in the ipsilateral hippocampal CA1 region, and the rat brain group was detected at the same time. A variety of indicators, including superoxide dismutase activity, malondialdehyde, monoamine oxidase B, cholinesterase, acetylcholinesterase, tumor necrosis factor alpha, and interleukin 1. Results and conclusions: (1) in water maze experiment, the learning and memory ability of rats could be improved significantly in the water maze group, and the model group was compared with the control group in the hippocampus CA1 region. The length of long duration enhancement was significantly decreased (P0.05); (2) there was no significant difference between group 5 g/kg and model group, and the peak potential amplitude of group 20 g/kg group was significantly higher than that of model group and 5 g/kg group of tonifying kidney Yizi prescription, which could reduce the length of D- galactose combined with beta amyloid protein 25-35 on hippocampus CA1 region. The inhibitory effect of process enhancement to improve the plasticity of synaptic function; (3) the level of TNF - alpha and interleukin 1 (P0.05) was significantly reduced, and the activity of cholinyl acetyltransferase and superoxide dismutase (P0.05), the activity of acetylcholinesterase and the level of malondialdehyde (P0.05) were significantly increased; (4) the results suggested that The improvement of the learning and memory of the rat model of Alzheimer's disease may be related to the inhibition of the inflammatory reaction in the brain, the regulation of the cholinergic system, the antioxidation and so on.
【作者单位】：河南中医药大学第二附属医院脑病一区;
【基金】：郑州市科技发展计划项目(2010SFXM273)~~
【分类号】：R285.5;R-332

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