东北汉族群体19个Y-STR基因座多态性和东北亚地区各民族的聚类分析

发布时间：2018-06-04 19:05

本文选题：Y染色体 + 短串联重复序列　；参考：《重庆医学》2017年05期

【摘要】：目的研究东北汉族群体的19个Y-STR基因座的遗传多态性和东北亚15个群体间的遗传关系,探讨其法医学和群体遗传学研究的应用价值。方法采用Y-filer复合扩增体系对200例东北汉族无血缘关系的男性个体的19个Y-STR基因座进行扩增,应用ABI310分析仪进行基因分型,采用Arelquin3.11、Mega4.1和SPSS17.0软件进行群体间的AMOVA分析、聚类分析和多维标度分析。结果东北汉族群体的19个Y-STR基因座的基因多态性普遍较高,共检出200个单倍型,单倍型多态性在0.350 9(DYS391)~0.971 1(DYS385a/b),适合应用于法医学个体识别和亲权鉴定;东北亚15个群体间存在差异,遗传距离在0.000 9~0.643 2,群体间聚类分析和多维度分析结果与民族起源和迁徙史基本相一致。结论东北汉族群体的19个Y-STR基因座的基因多态性普遍较高,适合应用于法医学个体识别和亲权鉴定中。
[Abstract]:Objective to study the genetic polymorphism of 19 Y-STR loci in Northeast China Han population and the genetic relationship among 15 populations in Northeast Asia. Methods Y-filer multiplex amplification system was used to amplify 19 Y-STR loci of 200 unrelated male individuals from Northeast Han nationality. The genotyping was performed by ABI310 analyzer, and AMOVA analysis was performed by using Arelquin3.11 Mega 4.1 and SPSS17.0 software. Cluster analysis and multidimensional scale analysis. Results the polymorphism of 19 Y-STR loci in Northeast Han population was generally high. A total of 200 haplotypes were detected, and the haplotype polymorphism was found at 0.350 9(DYS391)~0.971 1 DYS385a / b / b, which was suitable for forensic individual identification and paternity identification, and there were differences among 15 populations in Northeast Asia. The genetic distance was 0.000 ~ 0.643 2. The results of cluster analysis and multi-dimensional analysis were consistent with the history of ethnic origin and migration. Conclusion the polymorphism of 19 Y-STR loci in Northeast Han population is generally high, which is suitable for forensic individual identification and paternity identification.
【作者单位】：齐齐哈尔医学院生物遗传教研室;延边大学法医学教研室;吉林省长春市公安局净月分局;
【分类号】：R394

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