清金化痰汤对慢性阻塞性肺疾病急性加重期模型大鼠肺组织Foxp3和RORγt表达的影响

发布时间：2018-06-05 01:38

本文选题：慢性阻塞性肺疾病急性加重期 + 气道黏液高分泌　；参考：《北京中医药大学学报》2016年12期

【摘要】：目的探讨清金化痰汤对慢性阻塞性肺疾病急性加重期(AECOPD)模型大鼠肺组织叉头翼状螺旋转录因子P3(Foxp3)、维甲酸相关孤核受体γt(RORγt)表达的影响。方法 40只SPF Wistar雄性大鼠随机分为正常组、模型组、清金化痰汤组、克拉霉素组。除正常组外,其余组采取气道滴注脂多糖(LPS)联合烟熏的方法建立AECOPD气道黏液高分泌模型,并同时连续30 d分别给予生理盐水、清金化痰汤(8.4 g/kg)、克拉霉素(50 mg/kg)灌胃。实验第31天,提取大鼠肺组织,制作病理切片,并采用免疫组化法检测肺组织中黏蛋白5AC(Muc5AC)、中性粒细胞弹性蛋白酶(NE)的表达,观察Foxp3和RORγt的表达,免疫印迹法检测肺组织中Foxp3和RORγt的蛋白表达。结果与正常组比较,模型组Foxp3表达量无明显差异(P0.05),RORγt、Muc5AC、NE表达显著升高,Foxp3/RORγt显著下降(P0.01);清金化痰汤组与模型组比较,Foxp3表达量无明显差异(P0.05),RORγt、Muc5AC、NE表达显著降低,Foxp3/RORγt显著升高(P0.01或P0.05)。清金化痰汤组与克拉霉素组比较,Muc5AC显著降低(P0.05)。结论清金化痰汤可显著下调模型大鼠肺组织中RORγt蛋白表达,上调Foxp3/RORγt,这可能是清金化痰汤调节AECOPD黏液高分泌的机制之一。
[Abstract]:Objective to investigate the effect of Qingjinhuatan decoction (QJHuatang) on the expression of p3Foxp3, a paracronal receptor 纬 t(ROR 纬 t, in the lung tissue of rats with acute exacerbation of chronic obstructive pulmonary disease (COPD). Methods 40 male SPF Wistar rats were randomly divided into normal group, model group, Qingjin Huatan decoction group and clarithromycin group. The model of airway mucus hypersecretion of AECOPD was established by injecting lipopolysaccharide (LPS) and fumigation into the airway of the other groups except the normal group. At the same time, normal saline, Qingjinhuatan decoction (8.4 g / kg) and clarithromycin (50 mg / kg) were administered intragastrically for 30 days at the same time. On the 31st day of the experiment, the lung tissues of rats were extracted, and pathological sections were made. The expression of mucin 5ACMuc5ACN, neutrophil elastase (nep) and the expression of Foxp3 and ROR 纬 t were detected by immunohistochemical method. The protein expression of Foxp3 and ROR 纬 t in lung tissue was detected by Western blot. Results there was no significant difference in the expression of Foxp3 between the model group and the normal group. The expression of ROR 纬 -muc5ACNE in the model group was significantly increased and the expression of Foxp3 / ROR 纬 -t was significantly decreased (P < 0.01), while there was no significant difference in the expression of Foxp3 between the Qingjinhuatan decoction group and the model group. The expression of P0.05ROR 纬 -Muc5ACNE in the model group was significantly lower than that in the model group, and the expression of Foxp3 / ROR 纬 -t increased significantly (P0.01 or P0.05A). Compared with clarithromycin group, Qingjin Huatan decoction group decreased P0.05AC significantly. Conclusion Qingjinhuatan decoction can significantly down-regulate the expression of ROR 纬 t protein and up-regulate the expression of Foxp3/ROR 纬 t in lung tissue of model rats, which may be one of the mechanisms of Qingjin Huatan decoction regulating the hypersecretion of AECOPD mucus.
【作者单位】：北京中医药大学东直门医院;北京大学人民医院;北京中医药大学;
【基金】：国家自然科学基金项目(No.81473655)
【分类号】：R285.5;R-332

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