ABO血型综合实验基因分型技术简化及其群体遗传分析拓展

发布时间：2018-06-05 03:13

本文选题：ABO血型 + 遗传学教学　；参考：《遗传》2017年05期

【摘要】：ABO血型是生活中最常见、运用最广泛的遗传性状之一。人类ABO血型由I~A、I~B和i3个复等位基因决定,它们负责编码不同的糖基转移酶,进而决定3种血红细胞表面抗原。ABO血型涉及复等位基因、基因互作、单核苷酸多态(SNP)、基因演化等多个关键知识内容,是理想的遗传学教学案例。本文以ABO血型为研究对象,对遗传学实验进行了创新与整合。首先,在分子遗传学模块中建立了新颖的ABO血型基因分型方法:基于SNP位点设计特异性引物,通过实时定量PCR鉴定基因型;其次,在群体遗传学模块中创新了基因演化的实验教学方法,开发群体遗传学软件,利用计算机模拟不同条件下ABO血型决定基因频率的演化趋势。这些教学改革举措旨在丰富遗传学实验内容,拓展教学手段,提高学习效率。
[Abstract]:ABO blood group is one of the most common and widely used genetic traits in life. The human ABO blood group is determined by the three complex alleles Igna Igna B and I, which encode different glycosyltransferases, which in turn determine that the three blood RBC surface antigen. ABO blood groups involve complex alleles and gene interaction. Single nucleotide polymorphisms (SNPs), gene evolution and many other key knowledge contents are ideal teaching cases of genetics. In this paper, the genetic experiment was innovated and integrated with ABO blood group. Firstly, a novel genotyping method of ABO blood group was established in molecular genetics module. Specific primers were designed based on SNP locus, and genotypes were identified by real-time quantitative PCR. In the module of population genetics, the experimental teaching method of gene evolution is innovated, the software of population genetics is developed, and the evolution trend of ABO blood group determining gene frequency under different conditions is simulated by computer. The aim of these teaching reform measures is to enrich the contents of genetics experiments, expand teaching methods and improve learning efficiency.
【作者单位】：复旦大学生命科学学院;复旦大学医学院;
【基金】：国家基础学科人才培养基金项目(编号:J1210012) 上海市教委本科教学质量与教学改革计划资助~~
【分类号】：R394

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