公驼枕腺分泌物对小鼠免疫功能的影响及抗肿瘤作用研究

发布时间：2018-06-06 19:46

本文选题：公驼枕腺分泌物 + 免疫抑制小鼠　；参考：《内蒙古农业大学》2012年硕士论文

【摘要】：目的：研究公驼枕腺分泌物(poll gland secretion, PGS)对免疫抑制小鼠免疫功能的影响及对荷瘤小鼠的抗肿瘤作用。方法：采用腹腔注射环磷酰胺法制造免疫抑制小鼠模型,运用S180细胞悬液腋窝皮下接种法构建小鼠荷瘤模型。对于免疫抑制模型实验小鼠,随机分为正常组,环磷酰胺模型对照组,PGS高(250mg/kg).中(25mg/kg).低(2.5mg/kg)剂量组,灌胃给予药物,每日1次,用药14d后处死小鼠。称量各组小鼠体重、计算免疫器官指数,并采用ELISA法检测血清中IL-6和TNF-α以及IgG和IgM的含量：采用碳粒廓清法测定各组小鼠单核巨噬细胞系统的吞噬功能。对于S180荷瘤小鼠模型,随机分成5组：肿瘤模型阴性对照组、PGS高、中、低剂量处理组(250mg/kg.25mg／kg口2.5mg/kg)及环磷酰胺阳性对照处理组,每只小鼠右前肢腋窝皮下分别接种0.2mL从荷瘤小鼠腹腔中取得的瘤细胞液(用生理盐水调整到含瘤细胞数1×107个／mL)。观察小鼠的状态及肿瘤体积大小,用药28d,于第29d处死小鼠,对其进行解剖,称量小鼠实体瘤重量,并计算抑瘤率,并用ELISA法检测IL-2、IL-6、TNF-α、IgG和IgM等相关免疫指标,对解剖所得实体瘤组织做HE染色石蜡切片,在显微镜下进行组织切片观察,采用MTT法检测PGS对S180细胞抑制率。结果：与环磷酰胺模型对照组相比,中、高剂量PGS能显著提高免疫抑制小鼠胸腺指数和脾脏指数(P0.05),显著提高血清中IL-6含量(P0.05)和IgG含量；高剂量PGS能显著提高小鼠血清TNF-α的含量(P0.05),低、中剂量PGS组小鼠血清中TNF-α含量亦有上升趋势：PGS高剂量组小鼠单核巨噬细胞吞噬能力显著高于环磷酰胺模型对照组小鼠；高剂量PGS对荷瘤小鼠的肿瘤生长具有一定的抑制作用,石蜡切片的结果也显示高剂量PGS具有一定的抑制肿瘤生长作用；同时,中高剂量PGS对提高荷瘤小鼠血清TNF-α和IL-2和IL-6等细胞因子也具有提升作用；对于荷瘤小鼠,IgG含量的变化不是很明显,而IgM含量则出现了逆向增长的情况,显示PGS对体液免疫中免疫球蛋白的作用比较复杂。结论：一定剂量PGS可明显增加免疫抑制小鼠免疫器官的相对重量,提高脏器指数,并可显著提高免疫抑制小鼠血清TNF-α和IL-6等细胞因子以及IgM和IgG含量。PGS对荷瘤小鼠肿瘤生长具有一定的抑制作用,抑制作用呈现较明显的量效关系：高剂量PGS对荷瘤小鼠肿瘤的生长具有较为显著的抑制作用,同时可显著提高荷瘤小鼠血清TNF-α、IL-2和IL-6等细胞因子水平。一定剂量的PGS对免疫抑制小鼠和荷瘤小鼠的特异性免疫和非特异性免疫均有增强作用。
[Abstract]:Aim: to study the effect of male camel occipital gland secretion poll gland secretion (PGSs) on immune function of immunosuppressive mice and its antitumor effect on tumor-bearing mice. Methods: the immunosuppressive mice model was established by intraperitoneal injection of cyclophosphamide and subcutaneous inoculation of S180 cell suspension. The immunosuppressive mice were randomly divided into normal control group and cyclophosphamide model control group with PGS of 250 mg / kg 路kg ~ (-1). In the middle of 25 mg / kg. The mice in the low dose group (2.5 mg / kg) were given intragastric administration once a day, and the mice were killed after 14 days of administration. The body weight, immune organ index, serum IL-6, TNF- 伪, IgG and IgM were measured by Elisa. The phagocytic function of mononuclear macrophage system in each group was determined by carbon particle clearance method. S180 tumor-bearing mice were randomly divided into 5 groups: the tumor model negative control group was treated with 250 mg / kg / kg 2.5 mg / kg PGS and the cyclophosphamide positive control group. Each mouse was subcutaneously inoculated with 0.2 mL tumor cell fluid (adjusted with normal saline to 1 脳 107 mLX) from the abdominal cavity of the tumor-bearing mice. The state and tumor volume of the mice were observed. The mice were killed on the 29th day after 28 days of administration. The mice were dissected and weighed, and the tumor inhibition rate was calculated. The immunological indexes such as IL-2mil, IL-6, TNF- 伪 IgG and IgM were detected by Elisa. The paraffin sections of solid tumor tissue were stained with HE and observed under microscope. The inhibitory rate of PGS on S180 cells was detected by MTT method. Results: compared with cyclophosphamide model control group, high dose PGS could significantly increase thymus index and spleen index of immunosuppressive mice (P 0.05), increase serum IL-6 content (P 0.05) and IgG content. The content of TNF- 伪 in the serum of mice was significantly increased by high dose PGS, and the content of TNF- 伪 in the serum of medium dose PGS group was significantly higher than that of cyclophosphamide model group. The level of TNF- 伪 in the serum of mice in the middle dose PGS group was also increased. The phagocytosis ability of mononuclear macrophages in the high dose group was significantly higher than that in the control group. High dose PGS could inhibit tumor growth in tumor-bearing mice, and paraffin section showed that high dose PGS could inhibit tumor growth. The medium and high dose of PGS also increased the cytokines such as TNF- 伪, IL-2 and IL-6 in tumor bearing mice, but the change of IgG content in tumor bearing mice was not obvious, but the IgM content increased inversely. The results showed that the effect of PGS on immunoglobulin in humoral immunity was complicated. Conclusion: a certain dose of PGS can significantly increase the relative weight of immune organs and increase the visceral index of immunosuppressive mice. Serum TNF- 伪 and IL-6 and IgM and IgG contents in immunosuppressive mice were significantly increased. PGS could inhibit tumor growth in tumor-bearing mice to some extent. The inhibitory effect showed a dose-effect relationship: high dose of PGS could significantly inhibit tumor growth in tumor-bearing mice and increase the levels of cytokines such as TNF- 伪, IL-2 and IL-6 in serum of tumor-bearing mice. A certain dose of PGS enhanced the specific and nonspecific immunity of immunosuppressive mice and tumor-bearing mice.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332;R285.5

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