不同时期H1N1甲型流感病毒H1血凝素蛋白的特征比较

发布时间：2018-06-07 13:56

本文选题：HN甲型流感病毒 + 血凝素蛋白　；参考：《南昌大学学报(医学版)》2016年05期

【摘要】：目的比较2007—2014年分离的H1N1甲型流感病毒H1血凝素蛋白与分离于1934年的H1N1甲型流感病毒H1血凝素蛋白的差异性,以了解近几年流行的H1N1甲型流感病毒H1血凝素蛋白的变异特征。方法从Genebank的基因数据库中获取16株H1N1甲型流感病毒H1血凝素蛋白的核苷酸和氨基酸序列,采用在线软件SignalP4.1预测信号肽,运用Clustal X,Bioedit等国际通用软件进行氨基酸和核苷酸的序列比对,计算核苷酸及氨基酸的同源性。在线软件Antheprot分析二级结构。结果 H1血凝素蛋白编码框架长约1701bp,编码含566个氨基酸组成的多肽。16株H1N1甲型流感病毒H1血凝素氨基酸同源性为92.4%~99.8%,核苷酸同源性为91.8%~99.6%。H1血凝素蛋白aa1-aa17区域为潜在信号肽,血凝素蛋白A1和A2裂解序列为位于aa324-aa330序列IQSR↓GLF。H1血凝素蛋白富含潜在α螺旋(25%~32%)、β折叠(27%~28%)和卷曲结构(23%~26%)等二级结构。2007—2014年的15株病毒的血凝素蛋白A1高变区位于羧基端aa278-aa321,有11个位点变异,变异率高达25%。参与二硫键形成的10个半胱氨酸位点未发生变异,3个受体结合区的氨基酸序列(PKTS、VLVLWAIHH和SRYSKKFK)比较保守,有2个序列出现毒力相关位点D222G突变。结论与1934年的H1N1甲型流感病毒H1血凝素蛋白比较,2007—2014年间分离H1N1甲型流感病毒H1血凝素蛋白的重要功能结构域的序列仍保守稳定,但在某些位点出现较高频率的替代变异。
[Abstract]:Objective to compare the difference of H1 hemagglutinin protein of H1N1 influenza A virus and H1N1 influenza A virus H1 hemagglutinin protein isolated from 2007 to 2014 in order to understand the variation of H1 hemagglutinin protein of H1N1 influenza A virus in recent years. Methods the nucleotide and amino acid sequences of H1 hemagglutinin protein of H1N1 influenza A virus were obtained from Genebank gene database. The signal peptide was predicted by online software SignalP4.1. The sequence alignment of amino acids and nucleotides was carried out by using Clustal XG Bioedit and other international software, and the homology of nucleotides and amino acids was calculated. The secondary structure is analyzed by online software Antheprot. Results the coding frame of H1 hemagglutinin protein was about 1701 BP, encoding 566 amino acids. The amino acid homology of H1 hemagglutinin encoding H1N1 influenza A virus H1 hemagglutinin was 92.4% and 99.8, and the nucleotide homology was 91.8%~99.6%.H1 hemagglutinin protein aa1-aa17 region, which was potential signal peptide. Hemagglutinin A 1 and A 2 cleavage sequences are located in the secondary structure of aa324-aa330 sequence IQSR IQSR GLF.H1 hemagglutinin protein rich in potential 伪 helix 2532T, 尾 folding 2728T) and crimp structure 23x2626.The high variation region of hemagglutinin A1 of 15 strains of virus from 2007 to 2014 is located in carboxylic acid. The base terminal aa278-aa321 has 11 loci. The mutation rate is as high as 25. The 10 cysteine sites involved in the formation of disulfide bonds were not mutated, and the amino acid sequences of the three receptor binding regions, PKTS VLVLWAIHH and SRYSKKFK, were conserved, and two of them showed D222G mutations in virulence related sites. Conclusion compared with the H1 hemagglutinin protein of H1N1 influenza A virus in 1934, the sequence of important functional domain of H1 hemagglutinin protein isolated from H1N1 A influenza virus from 2007 to 2014 is still conserved and stable, but there is a high frequency of substitution variation at some sites.
【作者单位】：南昌大学公共卫生学院;南昌大学抚州医学院;南昌大学基础医学院微生物学教研室;
【分类号】：R373.13

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